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| Description
| - To ensure that egg-containing products, such as dried eggs and egg pasta, conform to the technological and legislative requirements for egg content, methods are needed to determine the amount of cholesterol in such products. The conventional approach, direct saponification and hexane extraction followed by cholesterol determination by gas chromatography coupled to a flame ionization detector (GC-FID), is very time consuming. Therefore, we developed a rapid method based on direct analysis in real time coupled to time-of-flight mass spectrometry (DART-TOF MS). Samples were prepared simply by solvent extraction followed by extract filtration. The optimization of certain parameters, including the solvent used and DART ionization gas temperature, had a pronounced effect on the intensities of the produced ions. In particular, the molecular and dehydrated ions of cholesterol and its deuterated analogue, cholesterol 2,2,3,4,4,6-d6, which was used as an internal standard. For the developed method, limits of detection and quantification were 0.03 and 0.05 mg g-1, respectively. The results of the real samples were compared to those obtained using the conventional approach (LOD= 0.002 mg g-1 and LOQ= 0.05 mg g-1), and it was found that, although the results obtained using the conventional approach were more accurate, our developed method is much simpler and faster, where the time was dramatically reduced by 87% for executing a screening analysis.
- To ensure that egg-containing products, such as dried eggs and egg pasta, conform to the technological and legislative requirements for egg content, methods are needed to determine the amount of cholesterol in such products. The conventional approach, direct saponification and hexane extraction followed by cholesterol determination by gas chromatography coupled to a flame ionization detector (GC-FID), is very time consuming. Therefore, we developed a rapid method based on direct analysis in real time coupled to time-of-flight mass spectrometry (DART-TOF MS). Samples were prepared simply by solvent extraction followed by extract filtration. The optimization of certain parameters, including the solvent used and DART ionization gas temperature, had a pronounced effect on the intensities of the produced ions. In particular, the molecular and dehydrated ions of cholesterol and its deuterated analogue, cholesterol 2,2,3,4,4,6-d6, which was used as an internal standard. For the developed method, limits of detection and quantification were 0.03 and 0.05 mg g-1, respectively. The results of the real samples were compared to those obtained using the conventional approach (LOD= 0.002 mg g-1 and LOQ= 0.05 mg g-1), and it was found that, although the results obtained using the conventional approach were more accurate, our developed method is much simpler and faster, where the time was dramatically reduced by 87% for executing a screening analysis. (en)
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| Title
| - DART mass spectrometry for rapid screening and quantitative determination of cholesterol in egg pasta
- DART mass spectrometry for rapid screening and quantitative determination of cholesterol in egg pasta (en)
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| skos:prefLabel
| - DART mass spectrometry for rapid screening and quantitative determination of cholesterol in egg pasta
- DART mass spectrometry for rapid screening and quantitative determination of cholesterol in egg pasta (en)
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| skos:notation
| - RIV/60461373:22330/14:43897133!RIV15-MZE-22330___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/14:43897133
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - GC-FID; egg pasta; cholesterol; ambient mass spectrometry; direct analysis in real time (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Mass Spectrometry
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Ševčík, Rudolf
- Rajchl, Aleš
- Čížková, Helena
- Grégrová, Adéla
- Al-Balaa, Dania
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
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| http://localhost/t...ganizacniJednotka
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