About: Peptide mass mapping as an effective tool for historical mortar analysis

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About: Peptide mass mapping as an effective tool for historical mortar analysis Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://ac.els-cdn.com/S0950061813008994/1-s2.0-S0950061813008994-main.pdf?_tid=5723656e-97d0-11e3-978f-00000aacb361&acdnat=1392640862_a75bc3432e7e36340526b27722def713
Description	Recently, peptide mass mapping using MALDI?TOF MS (Matrix-Assisted Laser Desorption/Ionization ? Time of Flight Mass Spectrometry) was shown to be an effective tool for protein analysis in such challenging insoluble materials as historical mortars. The first aim of this study was to investigate the influence of ageing on the performance of peptide mass mapping analysis using MALDI?TOF MS. Therefore, analyses were performed on model mortar samples with different compositions after 5 years of ageing. The second goal of this work was to analyse peptide fragments using LC?MS/MS (Liquid Chromatography coupled to tandem Mass Spectrometry), which represents a new approach. The use of this method led to obtaining the sequence information that allowed for unambiguous identification of the proteins present. Our study proved the complementarity of both approaches; therefore, their combination is highly beneficial. Recently, peptide mass mapping using MALDI?TOF MS (Matrix-Assisted Laser Desorption/Ionization ? Time of Flight Mass Spectrometry) was shown to be an effective tool for protein analysis in such challenging insoluble materials as historical mortars. The first aim of this study was to investigate the influence of ageing on the performance of peptide mass mapping analysis using MALDI?TOF MS. Therefore, analyses were performed on model mortar samples with different compositions after 5 years of ageing. The second goal of this work was to analyse peptide fragments using LC?MS/MS (Liquid Chromatography coupled to tandem Mass Spectrometry), which represents a new approach. The use of this method led to obtaining the sequence information that allowed for unambiguous identification of the proteins present. Our study proved the complementarity of both approaches; therefore, their combination is highly beneficial. (en)
Title	Peptide mass mapping as an effective tool for historical mortar analysis Peptide mass mapping as an effective tool for historical mortar analysis (en)
skos:prefLabel	Peptide mass mapping as an effective tool for historical mortar analysis Peptide mass mapping as an effective tool for historical mortar analysis (en)
skos:notation	RIV/60461373:22330/14:43895696!RIV15-MSM-22330___
http://linked.open...avai/riv/aktivita	S
http://linked.open...avai/riv/aktivity	S
http://linked.open...iv/cisloPeriodika	January
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Šantrůček, Jiří Hynek, Radovan Kučková, Štěpánka Crhová, Michaela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	36081
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/14:43895696
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	LC-MS/MS; MALDI-TOF MS; Peptide mass mapping method; Ageing; Protein additive; Historical mortar (en)
http://linked.open.../riv/klicoveSlovo	Peptide mass mapping method Protein additive LC-MS/MS Ageing Historical mortar MALDI-TOF MS
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[D4254669FBD0]
http://linked.open...i/riv/nazevZdroje	Construction and Building Materials
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	50
http://linked.open...iv/tvurceVysledku	Crhová, Michaela Hynek, Radovan Šantrůček, Jiří Hrdličková Kučková, Štěpánka
http://linked.open...ain/vavai/riv/wos	000330489200026
issn	0950-0618
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.conbuildmat.2013.09.059
http://localhost/t...ganizacniJednotka	22330

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