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Description
| - Preparations with elicitation activity were obtained from the mycelium of Leptosphaeria maculans, a fungal pathogen of oilseed rape (Brassica napus). Crude delipidated and deproteinized extract from fungal cell walls induced expression of pathogenesis related gene 1 (PR1), hydrogen peroxide accumulation, and enhanced resistance of B. napus plants toward infection by L. maculans. Elicitation activity significantly decreased after treatment of a crude extract with glucanase. Monosaccharide composition analysis of a crude extract purified by ion-exchange chromatography revealed glucose (similar to 58 mol %), mannose (similar to 22 mol %), and galactose (similar to18 mol %) as the major sugars. FT-IR and NMR spectra confirmed the presence of both carbohydrate and polypeptide components in the purified product. Correlation NMR experiments defined trisaccharide bound to O-3 of serine residue alpha-D-Glcp-(1-2)-beta-D-Galf-(1-6)-alpha-D-Manp-(1-3)-L-Ser. Terminal alpha-D-Glcp and (1-6)-beta-Dglucan were also detected. The obtained results strongly support the conclusion that these carbohydrates induce defense response in Brassica napus plants.
- Preparations with elicitation activity were obtained from the mycelium of Leptosphaeria maculans, a fungal pathogen of oilseed rape (Brassica napus). Crude delipidated and deproteinized extract from fungal cell walls induced expression of pathogenesis related gene 1 (PR1), hydrogen peroxide accumulation, and enhanced resistance of B. napus plants toward infection by L. maculans. Elicitation activity significantly decreased after treatment of a crude extract with glucanase. Monosaccharide composition analysis of a crude extract purified by ion-exchange chromatography revealed glucose (similar to 58 mol %), mannose (similar to 22 mol %), and galactose (similar to18 mol %) as the major sugars. FT-IR and NMR spectra confirmed the presence of both carbohydrate and polypeptide components in the purified product. Correlation NMR experiments defined trisaccharide bound to O-3 of serine residue alpha-D-Glcp-(1-2)-beta-D-Galf-(1-6)-alpha-D-Manp-(1-3)-L-Ser. Terminal alpha-D-Glcp and (1-6)-beta-Dglucan were also detected. The obtained results strongly support the conclusion that these carbohydrates induce defense response in Brassica napus plants. (en)
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Title
| - Cell wall components of Leptosphaeria maculans enhance the resistance of Brassica napus
- Cell wall components of Leptosphaeria maculans enhance the resistance of Brassica napus (en)
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skos:prefLabel
| - Cell wall components of Leptosphaeria maculans enhance the resistance of Brassica napus
- Cell wall components of Leptosphaeria maculans enhance the resistance of Brassica napus (en)
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skos:notation
| - RIV/60461373:22330/13:43896603!RIV14-MZE-22330___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA522/08/1581), P(QH81201), S, Z(MSM6046137305)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/13:43896603
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - resistance; Brassica napus; Leptoshaeria maculans; oligosaccharide; elicitor (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Journal of Agricultural and Food Chemistry
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Synytsya, Andriy
- Čopíková, Jana
- Burketová, Lenka
- Šašek, Vladimír
- Dinh Kim, Phuong
- Valentová, Olga
- Jindřichová, Barbora
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://bibframe.org/vocab/doi
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http://localhost/t...ganizacniJednotka
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