| Attributes | Values |
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| rdf:type
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| Description
| - The aim of this work is the preparation of transgenic plant resistant to both organic and inorganic pollutants. Firstly transgenic plants containing bacterial bphC gene were prepared. The bphC gene is coding for 2,3- dihydroxybiphenyl-1,2-dioxygenase cleaving the biphenyl ring to form a product which could be further metabolized. The presence of bphC gene in first and second filial generation of transgenic plants was detected on the DNA and RNA level using PCR or RT-PCR methods. Transgenic plants contain the bphC gene in fusion with genes for detection markers GUS (gene for beta-glucuronidase) and LUC (gene for luciferase) and also bphC gene with histidine tail. The expression of the bphC/GUS and the bphC/LUC genes in plants was tested by histochemical assay proving the expression of detection markers. Also the expression of the bphC/His gene was verified after purification of the enzyme by affinity chromatography followed by Western blot and immunochemical assay. The expression level differed among transgenic lines. Based on results of degradation experiment with 2,3-dihydroxybiphenyl, the transgenic lines G1, H1 and L1 were transformed by plasmid carrying yeast methallothionein gene CUP in fusion with histidine tail. The methallothioneins are known for their ability to bind heavy metals, histidine tail is other binding domain enhancing the effect. The transformation of transgenic plants was mediated by Agrobacterium tumefaciens.Transgenic plants containing both bphC and CUP gene were observed and the transcription of genes was confirmed using transient expression as well as permanent transformation.
- The aim of this work is the preparation of transgenic plant resistant to both organic and inorganic pollutants. Firstly transgenic plants containing bacterial bphC gene were prepared. The bphC gene is coding for 2,3- dihydroxybiphenyl-1,2-dioxygenase cleaving the biphenyl ring to form a product which could be further metabolized. The presence of bphC gene in first and second filial generation of transgenic plants was detected on the DNA and RNA level using PCR or RT-PCR methods. Transgenic plants contain the bphC gene in fusion with genes for detection markers GUS (gene for beta-glucuronidase) and LUC (gene for luciferase) and also bphC gene with histidine tail. The expression of the bphC/GUS and the bphC/LUC genes in plants was tested by histochemical assay proving the expression of detection markers. Also the expression of the bphC/His gene was verified after purification of the enzyme by affinity chromatography followed by Western blot and immunochemical assay. The expression level differed among transgenic lines. Based on results of degradation experiment with 2,3-dihydroxybiphenyl, the transgenic lines G1, H1 and L1 were transformed by plasmid carrying yeast methallothionein gene CUP in fusion with histidine tail. The methallothioneins are known for their ability to bind heavy metals, histidine tail is other binding domain enhancing the effect. The transformation of transgenic plants was mediated by Agrobacterium tumefaciens.Transgenic plants containing both bphC and CUP gene were observed and the transcription of genes was confirmed using transient expression as well as permanent transformation. (en)
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| Title
| - Preparation of super-transgenic plants expressing both dioxygenase and metallothionein genes
- Preparation of super-transgenic plants expressing both dioxygenase and metallothionein genes (en)
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| skos:prefLabel
| - Preparation of super-transgenic plants expressing both dioxygenase and metallothionein genes
- Preparation of super-transgenic plants expressing both dioxygenase and metallothionein genes (en)
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| skos:notation
| - RIV/60461373:22330/12:43893693!RIV13-GA0-22330___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GPP501/12/P521), P(LH12087), S
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/12:43893693
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - phytoremediation; metallothionein; BphC; transgenic plants (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - 5th International Symposium on Biosorption and Bioremediation
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Fišer, Jan
- Macek, Tomáš
- Macková, Martina
- Nováková, Martina
- Viktorová, Jitka
- Neumannová, E.
- Bečvářová, Zuzana
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
| |
| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - Vysoká škola chemicko-technologická v Praze
|
| https://schema.org/isbn
| |
| http://localhost/t...ganizacniJednotka
| |
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