About: Study of antimicrobial peptide induction in Brassica napus

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Development of cheap, effective and environmentally friendly protection of industrial plants is a key problem of mankind, where using of antimicrobial peptides could be a reasonable solution. As a source for isolation of these peptides canola (Brassica napus) was chosen, which was treated by functional analog of salicylic acid - benzothiadiazole (BTH) - to stimulate the immune system. To isolate the responsible antimicrobial peptides, crude buffer extract was obtained. Next steps were precipitation, centrifugation and molecular weight filtration. The further isolation procedure comprised hexane extraction, SPE - C18 (Solid Phase Extraction) to separate hydrophobic and hydrophilic fractions and reversed-phase chromatography. Antimicrobial activity was tested by diffusion method. Fractions were tested on gram-positive and gram-negative bacteria and on yeast Candida scotii. Development of cheap, effective and environmentally friendly protection of industrial plants is a key problem of mankind, where using of antimicrobial peptides could be a reasonable solution. As a source for isolation of these peptides canola (Brassica napus) was chosen, which was treated by functional analog of salicylic acid - benzothiadiazole (BTH) - to stimulate the immune system. To isolate the responsible antimicrobial peptides, crude buffer extract was obtained. Next steps were precipitation, centrifugation and molecular weight filtration. The further isolation procedure comprised hexane extraction, SPE - C18 (Solid Phase Extraction) to separate hydrophobic and hydrophilic fractions and reversed-phase chromatography. Antimicrobial activity was tested by diffusion method. Fractions were tested on gram-positive and gram-negative bacteria and on yeast Candida scotii. (en)
Title	Study of antimicrobial peptide induction in Brassica napus Study of antimicrobial peptide induction in Brassica napus (en)
skos:prefLabel	Study of antimicrobial peptide induction in Brassica napus Study of antimicrobial peptide induction in Brassica napus (en)
skos:notation	RIV/60461373:22330/11:43892577!RIV12-MSM-22330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA522/09/1693), P(GD305/09/H008), Z(MSM6046137305)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Neubauerová, Tereza Macková, Martina Macek, Tomáš Shevchenko, Irina
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	233029
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/11:43892577
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Induced resistance; Antimicrobial peptides; Brassica napus (en)
http://linked.open.../riv/klicoveSlovo	Brassica napus Antimicrobial peptides Induced resistance
http://linked.open...ontrolniKodProRIV	[8B550CF95765]
http://linked.open...v/mistoKonaniAkce	Praha
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	Collection Symposium Series: Biologically Active Peptides
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	The role of antimicrobial peptides in plant defense against pahogenic microorganisms Preparation, biotransformation, and optimalization of compounds with antitumor and antimicrobial effects
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Macek, Tomáš Macková, Martina Neubauerová, Tereza Shevchenko, Irina
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2011-04-27 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Teoretické základy potravinářských a biochemických technologií
number of pages	3 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Ústav organické chemie a biochemie AV ČR
https://schema.org/isbn	978-80-86241-44-9
http://localhost/t...ganizacniJednotka	22330

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