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Description
  • This preliminary study is aimed at a new approach in the protein analysis of hard tissues. Analytical methods that have been used for the identification of proteins in bones and teeth always require one critical step - demineralization. This chemical treatment is responsible for loss of proteins and it also negatively influences the possibility of protein quantification. The method of peptide mass mapping described in this paper facilitates a gentle releasing of peptides from the hard tissues without the loss of qualitative and quantitative information. Using this method led to identification of proteins from chicken thighbone, human jawbone and teeth, which proves the feasibility of this method.
  • This preliminary study is aimed at a new approach in the protein analysis of hard tissues. Analytical methods that have been used for the identification of proteins in bones and teeth always require one critical step - demineralization. This chemical treatment is responsible for loss of proteins and it also negatively influences the possibility of protein quantification. The method of peptide mass mapping described in this paper facilitates a gentle releasing of peptides from the hard tissues without the loss of qualitative and quantitative information. Using this method led to identification of proteins from chicken thighbone, human jawbone and teeth, which proves the feasibility of this method. (en)
Title
  • Novel Approach to Protein Analysis in Hard Tissues
  • Novel Approach to Protein Analysis in Hard Tissues (en)
skos:prefLabel
  • Novel Approach to Protein Analysis in Hard Tissues
  • Novel Approach to Protein Analysis in Hard Tissues (en)
skos:notation
  • RIV/60461373:22330/11:43892542!RIV12-MSM-22330___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GP203/07/P360), Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 216576
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22330/11:43892542
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Bone; Tooth; Hard Tissues; Mass Spectrometry (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • JP - Japonsko
http://linked.open...ontrolniKodProRIV
  • [A54B682D0E6B]
http://linked.open...i/riv/nazevZdroje
  • Journal of Surface Analysis
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 17
http://linked.open...iv/tvurceVysledku
  • Hynek, Radovan
  • Kodíček, Milan
  • Kučková, Štěpánka
  • Koník, Peter
  • Prchlíková, Radka
http://linked.open...n/vavai/riv/zamer
issn
  • 1341-1756
number of pages
http://localhost/t...ganizacniJednotka
  • 22330
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