About: Determination of cysteinyl leukotrienes in exhaled breath condensate: Method combining immunoseparation with LC-ESI-MS/MS

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About: Determination of cysteinyl leukotrienes in exhaled breath condensate: Method combining immunoseparation with LC-ESI-MS/MS Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A rapid and precise method for the identification and quantification of cysteinyl leukotrienes (leukotriene C4, leukotriene D4 and leukotriene E4), essential markers of bronchial asthma, in exhaled breath condensate was developed. The protocol consists of immunoaffinity sepn. and a detection step, liq. chromatog. combined with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). In particular, the selected reaction monitoring mode was used for its extremely high degree of selectivity and the stable-isotope-diln. assay for its high precision of quantification. The developed method was characterized with a high precision (?7.7%, detd. as RSD), an acceptable accuracy (90.4-93.7%, detd. as recovery), a low limit of detection (?2 pg/mL EBC) and a low limit of quantification (?10 pg/mL EBC). It was compared to other simple, clin. appropriate combinations of pre-treatment methods (solid phase extn. and lyophilization) with LC/MS. A rapid and precise method for the identification and quantification of cysteinyl leukotrienes (leukotriene C4, leukotriene D4 and leukotriene E4), essential markers of bronchial asthma, in exhaled breath condensate was developed. The protocol consists of immunoaffinity sepn. and a detection step, liq. chromatog. combined with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). In particular, the selected reaction monitoring mode was used for its extremely high degree of selectivity and the stable-isotope-diln. assay for its high precision of quantification. The developed method was characterized with a high precision (?7.7%, detd. as RSD), an acceptable accuracy (90.4-93.7%, detd. as recovery), a low limit of detection (?2 pg/mL EBC) and a low limit of quantification (?10 pg/mL EBC). It was compared to other simple, clin. appropriate combinations of pre-treatment methods (solid phase extn. and lyophilization) with LC/MS. (en)
Title	Determination of cysteinyl leukotrienes in exhaled breath condensate: Method combining immunoseparation with LC-ESI-MS/MS Determination of cysteinyl leukotrienes in exhaled breath condensate: Method combining immunoseparation with LC-ESI-MS/MS (en)
skos:prefLabel	Determination of cysteinyl leukotrienes in exhaled breath condensate: Method combining immunoseparation with LC-ESI-MS/MS Determination of cysteinyl leukotrienes in exhaled breath condensate: Method combining immunoseparation with LC-ESI-MS/MS (en)
skos:notation	RIV/60461373:22330/11:43891926!RIV12-MZ0-22330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GD203/08/H032), P(NS10298), Z(MSM6046137301)
http://linked.open...iv/cisloPeriodika	23
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Lipovová, Petra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	193819
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/11:43891926
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	LC-MS; Lyophilization; Solid phase extraction; Immunoseparation; Exhaled breath condensate; Cysteinyl leukotriene (en)
http://linked.open.../riv/klicoveSlovo	Cysteinyl leukotriene Exhaled breath condensate Immunoseparation LC-MS Lyophilization Solid phase extraction
http://linked.open...odStatuVydavatele	BE - Belgické království
http://linked.open...ontrolniKodProRIV	[47B0BFE8986A]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography B
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Advanced catalytic processes and materials Monitoring of biorhythms of metabolites arachidonic acid by standardized methodology
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	879
http://linked.open...iv/tvurceVysledku	Kačer, Petr Kuzma, Marek Lipovová, Petra Fenclová, Zdeňka Lebedová, Jindřiška Pelclová, Daniela Syslová, Kamila Vilhanová, Beáta
http://linked.open...ain/vavai/riv/wos	000293722800007
http://linked.open...n/vavai/riv/zamer	Catalytic processes in chemistry and chemical technology
issn	1570-0232
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.jchromb.2011.06.004
http://localhost/t...ganizacniJednotka	22330

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