About: Determination of phytic acid and inositolphosphates in barley

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Phytic acid (PA) and lower inositolphosphates (InsPn) is the main storage form of phosphorus in grains or seeds. The content of PA and InsPn in different varieties of barley was analyzed by capillary isotachophoresis and online-coupled capillary isotachophoresis with CZE. The electrolytes (in demineralized water) for the isotachophoretic analysis consisted of 10mM HCl, 14mM glycylglycine, and 0.1% 2-hydroxyethylcellulose (leading) and 10mM citric acid (terminating). The optimized electrolytes for the online coupling isotachophoresis with zone electrophoresis analysis were mixtures of 5mM HCl, 7mM glycylglycine, and 0.1% 2-hydroxyethylcellulose (leading), 20mM citric acid, 10mM glycylglycine, and 0.1% 2-hydroxyethylcellulose (background) and 10mM citric acid (terminating). PA and all studied InsPn were separated within 25 min and detected by a conductivity detector. Simple sample preparation (acidic extraction), sufficient sensitivity, speed of analysis, and low running cost are important attributes of the electrophoretic methods. The method was used for the determination of PA and InsPn in barley varieties within an ongoing research project. Phytic acid (PA) and lower inositolphosphates (InsPn) is the main storage form of phosphorus in grains or seeds. The content of PA and InsPn in different varieties of barley was analyzed by capillary isotachophoresis and online-coupled capillary isotachophoresis with CZE. The electrolytes (in demineralized water) for the isotachophoretic analysis consisted of 10mM HCl, 14mM glycylglycine, and 0.1% 2-hydroxyethylcellulose (leading) and 10mM citric acid (terminating). The optimized electrolytes for the online coupling isotachophoresis with zone electrophoresis analysis were mixtures of 5mM HCl, 7mM glycylglycine, and 0.1% 2-hydroxyethylcellulose (leading), 20mM citric acid, 10mM glycylglycine, and 0.1% 2-hydroxyethylcellulose (background) and 10mM citric acid (terminating). PA and all studied InsPn were separated within 25 min and detected by a conductivity detector. Simple sample preparation (acidic extraction), sufficient sensitivity, speed of analysis, and low running cost are important attributes of the electrophoretic methods. The method was used for the determination of PA and InsPn in barley varieties within an ongoing research project. (en)
Title	Determination of phytic acid and inositolphosphates in barley Determination of phytic acid and inositolphosphates in barley (en)
skos:prefLabel	Determination of phytic acid and inositolphosphates in barley Determination of phytic acid and inositolphosphates in barley (en)
skos:notation	RIV/60461373:22330/11:43870050!RIV12-MSM-22330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QH91053), Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika	32
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Voldřich, Michal Václavíková, Eliška Ševčík, Rudolf Kvasnička, František Čopíková, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	193865
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/11:43870050
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Barley / Inositolphosphates / Isotachophoresis / Phytate (en)
http://linked.open.../riv/klicoveSlovo	Barley / Inositolphosphates / Isotachophoresis / Phytate
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[9DF993EF11DB]
http://linked.open...i/riv/nazevZdroje	Electrophoresis, 21
http://linked.open...in/vavai/riv/obor	GM
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Improvement of barley grain quality using donors with differentiated content of natural compounds with ambivalent nutritional effects
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	2011
http://linked.open...iv/tvurceVysledku	Vaculová, Kateřina Voldřich, Michal Čopíková, Jana Kvasnička, František Václavíková, Eliška Ševčík, Rudolf
http://linked.open...ain/vavai/riv/wos	000289639400019
http://linked.open...n/vavai/riv/zamer	Teoretické základy potravinářských a biochemických technologií
issn	0173-0835
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	22330

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