About: Preparation of the galactooligosaccharides in the batch reactor: Comparison of a commercial β-galactosidase and crude cell extracts from lactic acid bacteria

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About: Preparation of the galactooligosaccharides in the batch reactor: Comparison of a commercial β-galactosidase and crude cell extracts from lactic acid bacteria Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Galactooligosaccharides are produced from lactose by a glycosyl transfer of one or more D galactosyl units onto the D-galactose moiety of lactose catalysed by β-galactosidase. Many factors have influence on this reaction e.g. temperature, concentration of substrate and origin of enzyme. Enzymes from different sources produce different types of galactooligosaccharides. We used three different type of enzyme in this work. The one of them was from commercial source and the other two were isolated from bacteria in form of crude cell extract. Lactobacillus delbruckii ssp. bulgaricus and Streptococcus thermophilus were used as bacteria sources and Maxilact LX 5000 was used as commercial source. Lactose in buffer, ultrafiltration permeate and recombined whey were used as substrates. The concentration of enzymes was 0.5 U.mL-1 and concentration of used substrates was 50 g.L-1. The highest yield was obtained for both crude cell extracts but Lactobacillus delbruckii ssp. bulgaricus had lower yields for Galactooligosaccharides are produced from lactose by a glycosyl transfer of one or more D galactosyl units onto the D-galactose moiety of lactose catalysed by β-galactosidase. Many factors have influence on this reaction e.g. temperature, concentration of substrate and origin of enzyme. Enzymes from different sources produce different types of galactooligosaccharides. We used three different type of enzyme in this work. The one of them was from commercial source and the other two were isolated from bacteria in form of crude cell extract. Lactobacillus delbruckii ssp. bulgaricus and Streptococcus thermophilus were used as bacteria sources and Maxilact LX 5000 was used as commercial source. Lactose in buffer, ultrafiltration permeate and recombined whey were used as substrates. The concentration of enzymes was 0.5 U.mL-1 and concentration of used substrates was 50 g.L-1. The highest yield was obtained for both crude cell extracts but Lactobacillus delbruckii ssp. bulgaricus had lower yields for (en)
Title	Preparation of the galactooligosaccharides in the batch reactor: Comparison of a commercial β-galactosidase and crude cell extracts from lactic acid bacteria Preparation of the galactooligosaccharides in the batch reactor: Comparison of a commercial β-galactosidase and crude cell extracts from lactic acid bacteria (en)
skos:prefLabel	Preparation of the galactooligosaccharides in the batch reactor: Comparison of a commercial β-galactosidase and crude cell extracts from lactic acid bacteria Preparation of the galactooligosaccharides in the batch reactor: Comparison of a commercial β-galactosidase and crude cell extracts from lactic acid bacteria (en)
skos:notation	RIV/60461373:22330/10:00024389!RIV11-MSM-22330___
http://linked.open...avai/riv/aktivita	S Z
http://linked.open...avai/riv/aktivity	S, Z(MSM6046137305)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Pocedičová, Klára Čurda, Ladislav
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	281396
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/10:00024389
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	galactooligosaccharides, β-galactosidase, Streptococcus thermophilus; Lactobacillus delbruckii ssp. bulgaricus (en)
http://linked.open.../riv/klicoveSlovo	galactooligosaccharides Streptococcus thermophilus Lactobacillus delbruckii ssp. bulgaricus β-galactosidase
http://linked.open...ontrolniKodProRIV	[5682828FD751]
http://linked.open...v/mistoKonaniAkce	Praha
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	CHISA 2010
http://linked.open...in/vavai/riv/obor	GM
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Čurda, Ladislav Krausová, Jana Pocedičová Roz.hellerová, Klára Germain, B.
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2010-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Teoretické základy potravinářských a biochemických technologií
number of pages	6 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Process Engineering Publisher, Novosad, J.
https://schema.org/isbn	978-80-02-02210-7
http://localhost/t...ganizacniJednotka	22330

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