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  • Four microbial proteases (Alcalase, Flavourzyme, Neutrase and Protamex) were used for the preparation of whey protein hydrolysates. The aim of this research was to find out whether these hydrolysates can be used as a source of whey derived antioxidants. Hydrolyzed samples, including their unhydrolyzed protein solutions were tested by the ABTS (2,20-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) decolorization assay, by the total radical-trapping potential method and by the assay of liposomes peroxidation (fluorescence photometry). Antioxidant properties were enhanced by hydrolysis in most of cases. Alcalase hydrolysates were found as the most effective antioxidants as determined by ABTS assay (*50% of antioxidant activity at 0.1 mg ml-1 of hydrolysate in reaction) and fluorescence photometry. Liposomes were oxidized *50% less (1.1 lM of a-tocopherol equivalent) with Alcalase hydrolysates additive (at 5.85 mg ml-1 of hydrolysate in reaction). Hydrolysates did not inhibit the oxidat
  • Four microbial proteases (Alcalase, Flavourzyme, Neutrase and Protamex) were used for the preparation of whey protein hydrolysates. The aim of this research was to find out whether these hydrolysates can be used as a source of whey derived antioxidants. Hydrolyzed samples, including their unhydrolyzed protein solutions were tested by the ABTS (2,20-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) decolorization assay, by the total radical-trapping potential method and by the assay of liposomes peroxidation (fluorescence photometry). Antioxidant properties were enhanced by hydrolysis in most of cases. Alcalase hydrolysates were found as the most effective antioxidants as determined by ABTS assay (*50% of antioxidant activity at 0.1 mg ml-1 of hydrolysate in reaction) and fluorescence photometry. Liposomes were oxidized *50% less (1.1 lM of a-tocopherol equivalent) with Alcalase hydrolysates additive (at 5.85 mg ml-1 of hydrolysate in reaction). Hydrolysates did not inhibit the oxidat (en)
Title
  • Antioxidant properties of whey protein hydrolysates as measured by three methods.
  • Antioxidant properties of whey protein hydrolysates as measured by three methods. (en)
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  • Antioxidant properties of whey protein hydrolysates as measured by three methods.
  • Antioxidant properties of whey protein hydrolysates as measured by three methods. (en)
skos:notation
  • RIV/60461373:22330/10:00024313!RIV11-MSM-22330___
http://linked.open...avai/riv/aktivita
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  • Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika
  • 6
http://linked.open...vai/riv/dodaniDat
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  • 247152
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  • RIV/60461373:22330/10:00024313
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  • whey; protein; hydrolysis; ABTS; TRAP; liposom; antioxidant (en)
http://linked.open.../riv/klicoveSlovo
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  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [A6B82D2D0AD5]
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  • European Food Research and Technology
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  • 230
http://linked.open...iv/tvurceVysledku
  • Čurda, Ladislav
  • Dryáková, Adriena
  • Korhonen, Hannu
  • Marnila, Pertti
  • Pihlanto, Anne
http://linked.open...ain/vavai/riv/wos
  • 000275538100008
http://linked.open...n/vavai/riv/zamer
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  • 1438-2377
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  • 22330
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