About: Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food

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About: Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Food contamination caused by chemical hazards such as persistent organic pollutants (POPs) is a worldwide public health concern and requires continuous monitoring. The chromatography-based analysis methods for POPs are accurate and quite sensitive but they are time-consuming, laborious and expensive. Thus, there is a need for validated simplified screening tools, which are inexpensive, rapid, have automation potential and can detect multiple POPs simultaneously. In this study we developed a flow cytometry-based immunoassay (FCIA) using a color-encoded microbeads technology to detect benzo[a]pyrene (BaP) and other polycyclic aromatic hydrocarbons (PAHs) in buffer and food extracts as a starting point for the future development of rapid multiplex assays including other POPs in food, such as polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs). A highly sensitive assay for BaP was obtained with an IC50 of 0.3 μg L-1 using a monoclonal antibody (Mab22F12) against BaP, similar t Food contamination caused by chemical hazards such as persistent organic pollutants (POPs) is a worldwide public health concern and requires continuous monitoring. The chromatography-based analysis methods for POPs are accurate and quite sensitive but they are time-consuming, laborious and expensive. Thus, there is a need for validated simplified screening tools, which are inexpensive, rapid, have automation potential and can detect multiple POPs simultaneously. In this study we developed a flow cytometry-based immunoassay (FCIA) using a color-encoded microbeads technology to detect benzo[a]pyrene (BaP) and other polycyclic aromatic hydrocarbons (PAHs) in buffer and food extracts as a starting point for the future development of rapid multiplex assays including other POPs in food, such as polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs). A highly sensitive assay for BaP was obtained with an IC50 of 0.3 μg L-1 using a monoclonal antibody (Mab22F12) against BaP, similar t (en)
Title	Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food (en)
skos:prefLabel	Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food (en)
skos:notation	RIV/60461373:22330/10:00024297!RIV11-MSM-22330___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(7E08068)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Hajšlová, Jana Pulkrabová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	251178
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/10:00024297
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Polycyclic aromatic hydrocarbons; Flow cytometry; Persistent organic pollutants (en)
http://linked.open.../riv/klicoveSlovo	Persistent organic pollutants Flow cytometry Polycyclic aromatic hydrocarbons
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[1373F9DB9A64]
http://linked.open...i/riv/nazevZdroje	Anal.Chim.Acta
http://linked.open...in/vavai/riv/obor	GM
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	CONtaminants in Food and Feed: Inexpensive DEtectioN for Control of Exposure
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	672
http://linked.open...iv/tvurceVysledku	Hajšlová, Jana Pulkrabová, Jana Haasnoot, W. Meimaridou, A. Mintzas, D. Nielen, M. W. Noteboom, L.
http://linked.open...ain/vavai/riv/wos	000279851000003
issn	0003-2670
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	22330

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