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  • An indirect competitive enzyme immunoassay of Enterobacter sakazakii has been developed. The rabbit polyclonal antibodies to heat-labile or heat-stable antigen of the type strain E. sakazakii CNCTC 5739(T) were prepared for these purposes. The detection limits of enzyme immunoassays were within the range 0.6-14.4 x 10(5) cells/ml. Antibodies raised to heat-labile antigen were serotype-specific. Although they contain non-specific IgG fractions binding periplasmatic and cytosol proteins, the interactions of these immunoglobulins are not manifested under conditions of ELISA developed.
  • An indirect competitive enzyme immunoassay of Enterobacter sakazakii has been developed. The rabbit polyclonal antibodies to heat-labile or heat-stable antigen of the type strain E. sakazakii CNCTC 5739(T) were prepared for these purposes. The detection limits of enzyme immunoassays were within the range 0.6-14.4 x 10(5) cells/ml. Antibodies raised to heat-labile antigen were serotype-specific. Although they contain non-specific IgG fractions binding periplasmatic and cytosol proteins, the interactions of these immunoglobulins are not manifested under conditions of ELISA developed. (en)
Title
  • Characterisation of Antibodies for the Immunochemical Detection of Enterobacter sakazakii
  • Characterisation of Antibodies for the Immunochemical Detection of Enterobacter sakazakii (en)
skos:prefLabel
  • Characterisation of Antibodies for the Immunochemical Detection of Enterobacter sakazakii
  • Characterisation of Antibodies for the Immunochemical Detection of Enterobacter sakazakii (en)
skos:notation
  • RIV/60461373:22330/09:00022436!RIV10-MSM-22330___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika
  • Sp. Iss. 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
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http://linked.open...dnocenehoVysledku
  • 306739
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22330/09:00022436
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Enterobacter sakazakii; heat-labile antigen; IgG; ELISA; cross-reactivity (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [41D9D260621D]
http://linked.open...i/riv/nazevZdroje
  • Czech Journal of Food Sciences
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 27
http://linked.open...iv/tvurceVysledku
  • Škvor, Jiří
  • Hochel, Igor
http://linked.open...ain/vavai/riv/wos
  • 000274102900011
http://linked.open...n/vavai/riv/zamer
issn
  • 1212-1800
number of pages
http://localhost/t...ganizacniJednotka
  • 22330
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