About: Production of Biogenic Amines by Enterococci     Goto   Sponge   NotDistinct   Permalink

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Description
  • Enterococci were presented in all tested samples of raw cow milk (six samples) at the level 103 ? 105 CFU ml-1, fresh cheeses (five samples) at the level 102 ? 106 CFU g-1 and semi-hard cheeses (five samples) at the level 103 ? 105 CFU g-1. All 33 isolated Enterococcus strains were screened for decarboxylase activity by usage differential growth medium and 20 of them possessed tyrosine decarboxylase activity. A collection of eight strains with the strongest decarboxylase activity were identified by species specific PCR as E. faecium (Z3, Z4, Br4 and 6/4D strains) and E. faecalis (Ž4, 3/3C and 4/1A strains). Enterococcus spp. Z1 strain was not identified at the species level by used methods, but the genus was confirmed by PCR method. Their tyrosin decarboxylase activity was confirmed by TLC and detection of tdc gene. Z1, Z3 and Z4 strains showed also histidine decarboxylase activity on the differential growth medium with histidine, but this activity was evaluated by TLC as a false positive reaction of
  • Enterococci were presented in all tested samples of raw cow milk (six samples) at the level 103 ? 105 CFU ml-1, fresh cheeses (five samples) at the level 102 ? 106 CFU g-1 and semi-hard cheeses (five samples) at the level 103 ? 105 CFU g-1. All 33 isolated Enterococcus strains were screened for decarboxylase activity by usage differential growth medium and 20 of them possessed tyrosine decarboxylase activity. A collection of eight strains with the strongest decarboxylase activity were identified by species specific PCR as E. faecium (Z3, Z4, Br4 and 6/4D strains) and E. faecalis (Ž4, 3/3C and 4/1A strains). Enterococcus spp. Z1 strain was not identified at the species level by used methods, but the genus was confirmed by PCR method. Their tyrosin decarboxylase activity was confirmed by TLC and detection of tdc gene. Z1, Z3 and Z4 strains showed also histidine decarboxylase activity on the differential growth medium with histidine, but this activity was evaluated by TLC as a false positive reaction of (en)
Title
  • Production of Biogenic Amines by Enterococci
  • Production of Biogenic Amines by Enterococci (en)
skos:prefLabel
  • Production of Biogenic Amines by Enterococci
  • Production of Biogenic Amines by Enterococci (en)
skos:notation
  • RIV/60461373:22330/09:00022275!RIV10-MSM-22330___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(2B06048), Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika
  • 6
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 336756
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22330/09:00022275
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Enterococcus; decarboxylase; tyrosine; histidine; TLC; PCR (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [896CCF717313]
http://linked.open...i/riv/nazevZdroje
  • Czech Journal of Food Science
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 27
http://linked.open...iv/tvurceVysledku
  • Plocková, Milada
  • Kučerová, Kateřina
  • Tůma, Štěpán
  • Ondráčková, Iva
  • Svodová, Hana
http://linked.open...ain/vavai/riv/wos
  • 000274102900009
http://linked.open...n/vavai/riv/zamer
issn
  • 1212-1800
number of pages
http://localhost/t...ganizacniJednotka
  • 22330
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