About: Fatty acids, unusual glycophospholipids and DNA analyses of thermophilic bacteria isolated from hot springs     Goto   Sponge   NotDistinct   Permalink

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  • The composition of fatty acids in 12 strains of the genera Thermus, Meiothermus, Geobacillus and Alicyclobacillus was analyzed by gas chromatography?mass spectrometry. Major FAs found in the profiles included i-15:0, i-17:0, ai-15:0, i-16:0, 16:0, ai-17:0, together with some minor components. Branched FAs were predominant, forming more than 80% of all FAs measured. Fast atom bombardment-mass spectrometry was used for analysis of unusual glycophospholipids, i.e., acylglycosylcardiolipins from genera Geobacillus and Alicyclobacillus and 1-(hydroxy(2-(O-acylglycosyl-oxy)hexadecyloxy) phosphoryloxy) hexadecan-2-yl esters of C15?C17 acids from genera Thermus and Meiothermus. Cloning and preliminary sequence analysis of 16S rDNA showed that these isolates belong to the genera Thermus, Meiothermus, Geobacillus and Alicyclobacillus.
  • The composition of fatty acids in 12 strains of the genera Thermus, Meiothermus, Geobacillus and Alicyclobacillus was analyzed by gas chromatography?mass spectrometry. Major FAs found in the profiles included i-15:0, i-17:0, ai-15:0, i-16:0, 16:0, ai-17:0, together with some minor components. Branched FAs were predominant, forming more than 80% of all FAs measured. Fast atom bombardment-mass spectrometry was used for analysis of unusual glycophospholipids, i.e., acylglycosylcardiolipins from genera Geobacillus and Alicyclobacillus and 1-(hydroxy(2-(O-acylglycosyl-oxy)hexadecyloxy) phosphoryloxy) hexadecan-2-yl esters of C15?C17 acids from genera Thermus and Meiothermus. Cloning and preliminary sequence analysis of 16S rDNA showed that these isolates belong to the genera Thermus, Meiothermus, Geobacillus and Alicyclobacillus. (en)
Title
  • Fatty acids, unusual glycophospholipids and DNA analyses of thermophilic bacteria isolated from hot springs
  • Fatty acids, unusual glycophospholipids and DNA analyses of thermophilic bacteria isolated from hot springs (en)
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  • Fatty acids, unusual glycophospholipids and DNA analyses of thermophilic bacteria isolated from hot springs
  • Fatty acids, unusual glycophospholipids and DNA analyses of thermophilic bacteria isolated from hot springs (en)
skos:notation
  • RIV/60461373:22330/09:00022170!RIV10-MSM-22330___
http://linked.open...avai/riv/aktivita
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  • Z(MSM6046137305)
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  • 1
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  • 314741
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  • RIV/60461373:22330/09:00022170
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  • Thermus; Meiothermus; Geobacillus; Alicyclobacillus; Fatty acids; Glycophospholipids; FAB-MS; 16S rDNA analysis; Phylogenetic tree; Hot springs (en)
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  • JP - Japonsko
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  • [E3BFCD0BCA9E]
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  • 13
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  • Melzoch, Karel
  • Řezanka, Tomáš
  • Siříšťová, Lucie
http://linked.open...ain/vavai/riv/wos
  • 000261986300011
http://linked.open...n/vavai/riv/zamer
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  • 1431-0651
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  • 22330
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