About: Flow cytometry for age assessment of a yeast population and its application in beer fermentations

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	An expeditious method of yeast age estimation was developed based on selective bud scar staining (Alexa Fluor 488-labelled wheat-germ agglutinin) and subsequent fluorescence intensity measurement by flow cytometry. The calibration curve resulting from the cytometric determination of average bud scar fluorescence intensities vs. microscopically counted average bud scar numbers of the same cell populations showed a good correlation and allowed routine cell age estimation by flow cytometry. The developed method was applied for yeast age control in traditional batch and continuous beer fermentations. At the pitching rates used in industrial beer fermentations, our results support former findings by locating a gradient of increasing yeast age from the top to the bottom zone of the fermenter cone. The results also indicate that in continuous beer fermentation, the increasing bud scar fluorescence of immobilized cells could help to schedule the replacement of aged biomass, prior to loss of viability or d An expeditious method of yeast age estimation was developed based on selective bud scar staining (Alexa Fluor 488-labelled wheat-germ agglutinin) and subsequent fluorescence intensity measurement by flow cytometry. The calibration curve resulting from the cytometric determination of average bud scar fluorescence intensities vs. microscopically counted average bud scar numbers of the same cell populations showed a good correlation and allowed routine cell age estimation by flow cytometry. The developed method was applied for yeast age control in traditional batch and continuous beer fermentations. At the pitching rates used in industrial beer fermentations, our results support former findings by locating a gradient of increasing yeast age from the top to the bottom zone of the fermenter cone. The results also indicate that in continuous beer fermentation, the increasing bud scar fluorescence of immobilized cells could help to schedule the replacement of aged biomass, prior to loss of viability or d (en)
Title	Flow cytometry for age assessment of a yeast population and its application in beer fermentations Flow cytometry for age assessment of a yeast population and its application in beer fermentations (en)
skos:prefLabel	Flow cytometry for age assessment of a yeast population and its application in beer fermentations Flow cytometry for age assessment of a yeast population and its application in beer fermentations (en)
skos:notation	RIV/60461373:22330/09:00022139!RIV10-MSM-22330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA104/06/1418), Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Lehnert, Radek Brányik, Tomáš Baszczyňski, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	315255
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/09:00022139
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Aging; beer; bud scar; flow cytometry; staining; yeast. (en)
http://linked.open.../riv/klicoveSlovo	bud scar staining Aging beer flow cytometry yeast.
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[25B0BA1C8635]
http://linked.open...i/riv/nazevZdroje	Journal of the Institute of Brewing
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Continuous fermentation of alcohol-free beer
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	115
http://linked.open...iv/tvurceVysledku	Brányik, Tomáš Kuřec, Michal Baszczyňski, Martin Lehnert, Radek Mota, Andre Texeira, Jose Antonio
http://linked.open...ain/vavai/riv/wos	000273080000013
http://linked.open...n/vavai/riv/zamer	Teoretické základy potravinářských a biochemických technologií
issn	0046-9750
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	22330

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