About: The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus.

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About: The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Most retroviruses employ a frameshift mechanism during polyprotein synthesis to balance appropriate ratios of structural proteins and enzymes. To investigate the requirements for individual precursors in retrovirus assembly, we modified the polyprotein repertoire of Mason-Pfizer monkey virus (M-PMV) by mutating the frameshift sites to imitate the polyprotein organization of Rous sarcoma virus (Gag-Pro and Gag-Pro-Pol) or Human immunodeficiency virus (Gag and Gag-Pro-Pol). For the %22Rous-like%22 virus, assembly was impaired with no incorporation of Gag-Pro-Pol into particles and for the %22HIV-like%22 virus an altered morphogenesis was observed. A mutant expressing Gag and Gag-Pro polyproteins and lacking Gag-Pro-Pol assembled intracellular particles at a level similar to the wild-type. Gag-Pro-Pol polyprotein alone neither formed immature particles nor processed the precursor. All the mutants were non-infectious except the %22HIV-like%22, which retained fractional infectivity. Most retroviruses employ a frameshift mechanism during polyprotein synthesis to balance appropriate ratios of structural proteins and enzymes. To investigate the requirements for individual precursors in retrovirus assembly, we modified the polyprotein repertoire of Mason-Pfizer monkey virus (M-PMV) by mutating the frameshift sites to imitate the polyprotein organization of Rous sarcoma virus (Gag-Pro and Gag-Pro-Pol) or Human immunodeficiency virus (Gag and Gag-Pro-Pol). For the %22Rous-like%22 virus, assembly was impaired with no incorporation of Gag-Pro-Pol into particles and for the %22HIV-like%22 virus an altered morphogenesis was observed. A mutant expressing Gag and Gag-Pro polyproteins and lacking Gag-Pro-Pol assembled intracellular particles at a level similar to the wild-type. Gag-Pro-Pol polyprotein alone neither formed immature particles nor processed the precursor. All the mutants were non-infectious except the %22HIV-like%22, which retained fractional infectivity. (en)
Title	The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus. The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus. (en)
skos:prefLabel	The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus. The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus. (en)
skos:notation	RIV/60461373:22330/09:00022120!RIV10-MSM-22330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0508), P(1M0520), P(GESCO/06/E001), P(KAN200100801), P(KAN208240651), P(ME 904), Z(AV0Z40550506), Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika	384
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Kohoutová roz. Smékalová, Zdena Rumlová, Michaela Ruml, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	318568
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/09:00022120
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Ribosomal frameshift; Retrovirus; Assembly; Mason-Pfizer monkey virus; Capsid (en)
http://linked.open.../riv/klicoveSlovo	Retrovirus Mason-Pfizer monkey virus Assembly Capsid Ribosomal frameshift
http://linked.open...odStatuVydavatele	BE - Belgické království
http://linked.open...ontrolniKodProRIV	[0C3C146AAE44]
http://linked.open...i/riv/nazevZdroje	Virology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20904 New Antivirals and Antineoplastics Center for Applied Genomics Bioactive Biocompatible Surfaces and Novel Nanostructured Composites for Applications to Medicine and Drug Delivery Study of interactions of biological macromolecules and nanolayers with a view to research of polymer microfluidic biosensors and therapeutic nanoparticles Interactions betwen viral and host cell proteins: from purification of macromolecular complexes to high-resolution structures
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Pichová, Iva Ruml, Tomáš Rumlová, Michaela Hunter, Eric Kohoutová roz. Smékalová, Zdena Andreánský, Martin Sakalian, Michael
http://linked.open...ain/vavai/riv/wos	000263039200010
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství Teoretické základy potravinářských a biochemických technologií
issn	0042-6822
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	22330

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