About: Isoflavones in the Rutaceae family: Twenty selected representatives of the genera Citrus, Fortunella, Poncirus, Ruta and Severinia     Goto   Sponge   NotDistinct   Permalink

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  • Enzyme-linked immunosorbent assays in combination with semi-preparative high-performance liquid chromatography (HPLC) and analytical HPLC with mass spectroscopy in the selective ion monitoring mode were used for the determination of selected isoflavones, daidzein, genistein, biochanin A and their homologues, in 20 representatives of the Rutaceae family. Species belonging to five genera were studied, namely Citrus, Fortunella Poncirus, Ruta and Severinia. The enzyme immunoassays used were based on polyclonal antibodies raised against isoflavonoid conjugates with bovine serum albumin (BSA), namely biochanin A-7-BSA, daidzein-7-BSA, daidzein-4'-BSA, genistein-7-BSA and genistein-4'-BSA. Aglycones as well as glycosides were detected, and methoxyisoflavones appeared to be more abundant than hydoxyisoflavones. The content of individual isoflavonoids ranged from 0 to 2.6 mg/kg (dry weight); the sum of all measured substances reached up to 5.9 mg.
  • Enzyme-linked immunosorbent assays in combination with semi-preparative high-performance liquid chromatography (HPLC) and analytical HPLC with mass spectroscopy in the selective ion monitoring mode were used for the determination of selected isoflavones, daidzein, genistein, biochanin A and their homologues, in 20 representatives of the Rutaceae family. Species belonging to five genera were studied, namely Citrus, Fortunella Poncirus, Ruta and Severinia. The enzyme immunoassays used were based on polyclonal antibodies raised against isoflavonoid conjugates with bovine serum albumin (BSA), namely biochanin A-7-BSA, daidzein-7-BSA, daidzein-4'-BSA, genistein-7-BSA and genistein-4'-BSA. Aglycones as well as glycosides were detected, and methoxyisoflavones appeared to be more abundant than hydoxyisoflavones. The content of individual isoflavonoids ranged from 0 to 2.6 mg/kg (dry weight); the sum of all measured substances reached up to 5.9 mg. (en)
Title
  • Isoflavones in the Rutaceae family: Twenty selected representatives of the genera Citrus, Fortunella, Poncirus, Ruta and Severinia
  • Isoflavones in the Rutaceae family: Twenty selected representatives of the genera Citrus, Fortunella, Poncirus, Ruta and Severinia (en)
skos:prefLabel
  • Isoflavones in the Rutaceae family: Twenty selected representatives of the genera Citrus, Fortunella, Poncirus, Ruta and Severinia
  • Isoflavones in the Rutaceae family: Twenty selected representatives of the genera Citrus, Fortunella, Poncirus, Ruta and Severinia (en)
skos:notation
  • RIV/60461373:22330/08:00021388!RIV10-MSM-22330___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA525/03/0352), P(GA525/06/0864), S, Z(MSM6046070901)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 373379
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22330/08:00021388
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • immunoassay; HPLC-MS; daidzein; genistein; biochanin (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [1D7D5ED7E9BB]
http://linked.open...i/riv/nazevZdroje
  • Phytochemical Analysis
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 19
http://linked.open...iv/tvurceVysledku
  • Kokoška, Ladislav
  • Lapčík, Oldřich
  • Koblovská, Radka
  • Vítková, Michaela
  • Macková, Zuzana
  • Klejdus, Borivoj
http://linked.open...ain/vavai/riv/wos
  • 000253340200008
http://linked.open...n/vavai/riv/zamer
issn
  • 0958-0344
number of pages
http://localhost/t...ganizacniJednotka
  • 22330
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