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| Description
| - Reactive oxygen species (ROS), e.g., hydrogen peroxide, are aerobic cellular by products, where excess oxidatively damages DNA, proteins and lipids. This is of particular concern regarding carcinogenesis. The Fenton reaction generates ROS. Several reports suggest haemoglobin-containing heme-iron, like other iron-rich substances, produce ROS in vitro due to this catalyst. As haemoglobin has been considered a villain, playing a negative role for human health in vitro, this potentially damaging effect on cellular DNA requires clarification in vivo. Here, we show that under the coexistence of haemoglobin derivatives and heme with hydrogen peroxide, DNA did not damage. This inhibition was confirmed in cell culture using a single cell gel electrophoresis assay, comet assay, proving haemoglobin has catalase activity. These results dispute prior findings that in vitro haemoglobin generates ROS, which damages DNA-inducing carcinogenesis. In fact, haemoglobin delays or inhibits carcinogenesis caused from DNA d
- Reactive oxygen species (ROS), e.g., hydrogen peroxide, are aerobic cellular by products, where excess oxidatively damages DNA, proteins and lipids. This is of particular concern regarding carcinogenesis. The Fenton reaction generates ROS. Several reports suggest haemoglobin-containing heme-iron, like other iron-rich substances, produce ROS in vitro due to this catalyst. As haemoglobin has been considered a villain, playing a negative role for human health in vitro, this potentially damaging effect on cellular DNA requires clarification in vivo. Here, we show that under the coexistence of haemoglobin derivatives and heme with hydrogen peroxide, DNA did not damage. This inhibition was confirmed in cell culture using a single cell gel electrophoresis assay, comet assay, proving haemoglobin has catalase activity. These results dispute prior findings that in vitro haemoglobin generates ROS, which damages DNA-inducing carcinogenesis. In fact, haemoglobin delays or inhibits carcinogenesis caused from DNA d (en)
- Reakční formy kyslíku (ROS), např. peroxid vodíku jsou aerobní buněčné produkty, jejichž přebytek oxidateivně poškozuje DNA, bílkoviny a lipidy. Bylo prokázáno, že při současné koexistenci hemových barviv a peroxidu vodíku DNA není poškozena. Tato inhibice byla potvrzena použitímpoužitím jednoduché gelové elektroforézy, comet assay, dokazující, že haemoglobin má katalázovou aktivitu. (cs)
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| Title
| - Evaluation of DNA damage induced with reactive oxygen and inhibitory effect of hem protein on its damage
- Vyhodnocení poškození DNA vyvolané reakcí kyslíku a inhibiční efekt hemových bílkoviny na toto poškození (cs)
- Evaluation of DNA damage induced with reactive oxygen and inhibitory effect of hem protein on its damage (en)
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| skos:prefLabel
| - Evaluation of DNA damage induced with reactive oxygen and inhibitory effect of hem protein on its damage
- Vyhodnocení poškození DNA vyvolané reakcí kyslíku a inhibiční efekt hemových bílkoviny na toto poškození (cs)
- Evaluation of DNA damage induced with reactive oxygen and inhibitory effect of hem protein on its damage (en)
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| skos:notation
| - RIV/60461373:22330/08:00021070!RIV09-MSM-22330___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/08:00021070
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Haemoglobin; Hydrogen peroxide; Comet assay; DNA damage; HL-60 (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Fleischwirtschaft International
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Pipek, Petr
- Yonehara, Makiko
- Izumimoto, Masatoshi
- Ly, Ei
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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