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| rdf:type
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| Description
| - Chemická modifikace postranních řetězců aminokyselin detekovaná hmotnostní spektrometií může odhalit alespoň částečné informace o 3D struktuře proteinů. V této práci jsme testovali diethylpyrokarbonát, jakožto činidlo běžně užívané k modifikaci histidinu. Vhodné podmínky pro reakci a detekci modifikovaných aminokyselin byly testovány na naiotensinu II. Studovali jsme modifikaci několika modelových proteinů se známou prostorovou strukturou (insulin, cytochrom c, lysozym a lidský sérový albumin). Naše výsledky ukázali, že povrchová dostupnost aminokyselinových zbytků je nutnou, nikoliv však postačující podmínkou pro reaktivitu; mikroprostředí postranních řetězců a dynamika proteinové struktury rovněž ovlivňují reaktivitu zbytků. Nicméně detekce modifikovaných zbytků může být brána jako důkaz jejich povrchové dostupnosti a přímého kontaktu s molekulami rozpuštědla. (cs)
- The chemical modification of amino acid side-chains followed by mass spectrometric detection can reveal at least partial information about the 3-D structure of proteins. In this work we tested diethylpyrocarbonate, as a common histidyl modification agent, for this purpose. Appropriate conditions for the reaction and detection of modified amino acids were developed using angiotensin II as a model peptide. We studied the modification of several model proteins with a known spatial arrangement (insulin, cytochrome c, lysozyme and human serum albumin). Our results revealed that the surface accessibility of residues is a necessary, although in itself insufficient, condition for their reactivity; the microenvironment of side-chains and the dynamics of protein structure also affect the ability of residues to react. However the detection of modified residues can be taken as proof of their surface accessibility, and of direct contact with solvent molecules.
- The chemical modification of amino acid side-chains followed by mass spectrometric detection can reveal at least partial information about the 3-D structure of proteins. In this work we tested diethylpyrocarbonate, as a common histidyl modification agent, for this purpose. Appropriate conditions for the reaction and detection of modified amino acids were developed using angiotensin II as a model peptide. We studied the modification of several model proteins with a known spatial arrangement (insulin, cytochrome c, lysozyme and human serum albumin). Our results revealed that the surface accessibility of residues is a necessary, although in itself insufficient, condition for their reactivity; the microenvironment of side-chains and the dynamics of protein structure also affect the ability of residues to react. However the detection of modified residues can be taken as proof of their surface accessibility, and of direct contact with solvent molecules. (en)
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| Title
| - Reactivity of histidine and lysine side-chains with diethylpyrocarbonate - A method to identify surface exposed residues in proteins
- Reaktivita histidinových a lysinových postranních řetězců s diethylpyrokarbonátem - metoda pro identifikaci povrchově dostupných zbytků v proteinech (cs)
- Reactivity of histidine and lysine side-chains with diethylpyrocarbonate - A method to identify surface exposed residues in proteins (en)
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| skos:prefLabel
| - Reactivity of histidine and lysine side-chains with diethylpyrocarbonate - A method to identify surface exposed residues in proteins
- Reaktivita histidinových a lysinových postranních řetězců s diethylpyrokarbonátem - metoda pro identifikaci povrchově dostupných zbytků v proteinech (cs)
- Reactivity of histidine and lysine side-chains with diethylpyrocarbonate - A method to identify surface exposed residues in proteins (en)
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| skos:notation
| - RIV/60461373:22330/08:00020949!RIV09-MSM-22330___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/08:00020949
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - histidine; diethylpyrocarbonate; mass spectrometry (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Biochemical and Biophysical Methods
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Kodíček, Milan
- Šantrůček, Jiří
- Hnízda, Aleš
- Šanda, Miloslav
- Strohalm, Martin
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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