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| rdf:type
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| Description
| - The carboxyl-terminal part of sexual adhesion glycoprotein -agglutinin (AG1Cp) was employed as an anchoring domain for surface display of short metal binding peptides of sequences N-Ser-(Gly-Cys-Gly-Cys-Pro-Cys-Gly-Cys)2-Gly-C (CP2 peptide) and N-Ser-(Gly-His-His-Pro-His)3-Gly-C (HP3 peptide) in the cell wall of potential biosorbent Saccharomyces cerevisiae. The constructed genetic fusions were efficiently expressed and found covalently attached to cell wall glucan and both O- and N-glycosylated. As a consequence of AG1Cp over-expression the nitrogen content of cell wall increased by 30%, which was reflected by increased sorption capacity of both isolated cell walls and intact cells. Comparing to parental cells, the surface display of mere anchoring domain doubled the amount of Cd2+ and Zn2+ adsorbed by modified biomass from solutions containing 10 to 200 M metal ions. Displayed CP2 and HP3 sequences showed selectivity towards Cd2+ and Zn2+, respectively. The fusion of CP2 peptide to AG1Cp sequence in
- The carboxyl-terminal part of sexual adhesion glycoprotein -agglutinin (AG1Cp) was employed as an anchoring domain for surface display of short metal binding peptides of sequences N-Ser-(Gly-Cys-Gly-Cys-Pro-Cys-Gly-Cys)2-Gly-C (CP2 peptide) and N-Ser-(Gly-His-His-Pro-His)3-Gly-C (HP3 peptide) in the cell wall of potential biosorbent Saccharomyces cerevisiae. The constructed genetic fusions were efficiently expressed and found covalently attached to cell wall glucan and both O- and N-glycosylated. As a consequence of AG1Cp over-expression the nitrogen content of cell wall increased by 30%, which was reflected by increased sorption capacity of both isolated cell walls and intact cells. Comparing to parental cells, the surface display of mere anchoring domain doubled the amount of Cd2+ and Zn2+ adsorbed by modified biomass from solutions containing 10 to 200 M metal ions. Displayed CP2 and HP3 sequences showed selectivity towards Cd2+ and Zn2+, respectively. The fusion of CP2 peptide to AG1Cp sequence in (en)
- C-koncová část glykoproteinu alfa-aglutininu (AGalfa1Cp) byla využita jako kotvící doména pro povrchovou expozici krátkých peptidů vážících těžké kovy na buněčné stěně potencionálního biosorbentu Saccharomyces cerevisiae. Byly vytvořeny genetické fúze AGalfa1Cp s peptidy o sekvencích N-Ser-(Gly-Cys-Gly-Cys-Pro-Cys-Gly-Cys)2-Gly-C (peptid CP2) a N-Ser-(Gly-His-His-Pro-His)3-Gly-C (peptid HP3). Připravené fúze byly účinně exprimovány v S. cerevisiae a lokalizovaly kovalentně zakotvené na glukan buněčné stěny, kde byly účinně O- and N-glykosylovány. V důsledku nadprodukce AGalfa1Cp došlo ke 30% zvýšení obsahu N v buněčné stěně a takto modifikované buňky akumulovaly v porovnání s parentálním kmenem dvojnásobná množství Cd2+ and Zn2+ z roztoků s obsahem 10 to 200 mikroM iontů těchto kovů. Peptidy CP2 and HP3, zakotvené na buněčné stěně, vykazovaly selektivitu k Cd2+ and Zn2+, v uvedeném pořadí. Expozice fúze CP2 s AGalfa1Cp zvýšila sorpční kapacitu pro Cd2+ o 30%, zatímco sorpce Zn2+ nebyla ovlivněna. Pov (cs)
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| Title
| - Biosorption of Cd2+ and Zn2+ by cell surface-engineered Saccharomyces cerevisiae.
- Biosorpce Cd2+ and Zn2+ buňkami Saccharomyces cerevisiae s modifikovaným buněčným povrchem. (cs)
- Biosorption of Cd2+ and Zn2+ by cell surface-engineered Saccharomyces cerevisiae. (en)
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| skos:prefLabel
| - Biosorption of Cd2+ and Zn2+ by cell surface-engineered Saccharomyces cerevisiae.
- Biosorpce Cd2+ and Zn2+ buňkami Saccharomyces cerevisiae s modifikovaným buněčným povrchem. (cs)
- Biosorption of Cd2+ and Zn2+ by cell surface-engineered Saccharomyces cerevisiae. (en)
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| skos:notation
| - RIV/60461373:22330/08:00019923!RIV09-MSM-22330___
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(1M0520), P(GP203/03/P095), Z(MSM6046137305)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/08:00019923
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - yeast biosorbent; alpha-agglutinin; cell surface-display; metal binding peptide (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - International Biodeterioration and Biodegradation
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Kotrba, Pavel
- Ruml, Tomáš
- Vinopal, Stanislav
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| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
| |
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