About: Detection of Campylobacter species in foods by indirect competitive ELISA using hen and rabbit antibodies

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About: Detection of Campylobacter species in foods by indirect competitive ELISA using hen and rabbit antibodies Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The competitive enzyme immunoassays for detection of Campylobacter jejuni, C. coli and C. fetus subsp. fetus have been developed. Rabbit and hen immunoglobulins were prepared for these purposes. The working conditions of ELISAs, such as the concentrations of immunoreactants, incubation temperatures and time, and the composition of the substrate have been established. The detection limits were in the range 5.0 104_3.2 106 cfu/ml. The application of chemiluminescent substrates did not result in any significant improvement of the assay's detectability and sensitivity. Prepared antibodies showed rather high specificity and crossreactivity profiles, and both rabbit and hen immunoglobulins were similar. Only IgY to C. jejuni cross-reacted with seven strains of C. jejuni and two other Campylobacter spp. The competitive enzyme immunoassays for detection of Campylobacter jejuni, C. coli and C. fetus subsp. fetus have been developed. Rabbit and hen immunoglobulins were prepared for these purposes. The working conditions of ELISAs, such as the concentrations of immunoreactants, incubation temperatures and time, and the composition of the substrate have been established. The detection limits were in the range 5.0 104_3.2 106 cfu/ml. The application of chemiluminescent substrates did not result in any significant improvement of the assay's detectability and sensitivity. Prepared antibodies showed rather high specificity and crossreactivity profiles, and both rabbit and hen immunoglobulins were similar. Only IgY to C. jejuni cross-reacted with seven strains of C. jejuni and two other Campylobacter spp. (en)
Title	Detection of Campylobacter species in foods by indirect competitive ELISA using hen and rabbit antibodies Detection of Campylobacter species in foods by indirect competitive ELISA using hen and rabbit antibodies (en)
skos:prefLabel	Detection of Campylobacter species in foods by indirect competitive ELISA using hen and rabbit antibodies Detection of Campylobacter species in foods by indirect competitive ELISA using hen and rabbit antibodies (en)
skos:notation	RIV/60461373:22330/07:43893203!RIV12-GA0-22330___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(GA102/03/0633), P(GA525/02/0287), S
http://linked.open...iv/cisloPeriodika	3-4
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Hochel, Igor
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	416513
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/07:43893203
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ELISA; cross-reactivity; polyclonal antibody; Campylobacter spp. (en)
http://linked.open.../riv/klicoveSlovo	cross-reactivity ELISA Campylobacter spp. polyclonal antibody
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[DA45BB6FD1D5]
http://linked.open...i/riv/nazevZdroje	Food and Agricultural Immunology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of screening ELISA and PCR methods for detection of Campylobacteria in foods Optical multisensors for detection of foodborne pathogens
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	18
http://linked.open...iv/tvurceVysledku	Steinhauserová, Iva Škvor, Jiří Hochel, Igor Viochna, David Slavíčková, Dana
http://linked.open...ain/vavai/riv/wos	000253264700001
issn	0954-0105
number of pages	17 (xsd:int)
http://localhost/t...ganizacniJednotka	22330

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