About: Characterization of rabbit antibodies for immunochemical detection of Yersinia enterocolitica

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About: Characterization of rabbit antibodies for immunochemical detection of Yersinia enterocolitica Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Rabbit IgG raised against whole cells of Yersinia enterocolitica O:3, Y. enterocolitica O:9 and against a group of pathogenic Y. enterocolitica strains (serotypes O:3, O:5,27, O:8. and O:9) were prepared. The antibody limiting titers were 1:2.0?105-7.5?105, 1:7.1?105-7.2?105, and 1:9.5?104-1.0?105, respectivelly. The immunoblotting analysis of Yersinia lipopolysacchides separated by SDS-PAGE shows that IgG against single serotype O:3 interacted with high molecular weight LPS of Y. enterocolitica O:3 whereas other antibodies were bound to low molecular weight LPS of Y. enterocolitica serotypes O:3, O:5,27, O:9 and serologicaly unspecified strain Y. enterocolitica (CNCTC Y 2/68). IgG against group of pathogenic serotypes also poorly interacted with low molecular weight LPS of Y. enterocolitica serotypes O:5, O:6,30, and O:10. The cross-reactivities of the antibodies with Y. pseudotuberculosis Ia and/or Y. rohdei b, d, e, f, i, which were observed by means of dot-blotting procedure using the whole bacterial cells as an antigen, is not caused by LPS of these bacteria. Rabbit IgG raised against whole cells of Yersinia enterocolitica O:3, Y. enterocolitica O:9 and against a group of pathogenic Y. enterocolitica strains (serotypes O:3, O:5,27, O:8. and O:9) were prepared. The antibody limiting titers were 1:2.0?105-7.5?105, 1:7.1?105-7.2?105, and 1:9.5?104-1.0?105, respectivelly. The immunoblotting analysis of Yersinia lipopolysacchides separated by SDS-PAGE shows that IgG against single serotype O:3 interacted with high molecular weight LPS of Y. enterocolitica O:3 whereas other antibodies were bound to low molecular weight LPS of Y. enterocolitica serotypes O:3, O:5,27, O:9 and serologicaly unspecified strain Y. enterocolitica (CNCTC Y 2/68). IgG against group of pathogenic serotypes also poorly interacted with low molecular weight LPS of Y. enterocolitica serotypes O:5, O:6,30, and O:10. The cross-reactivities of the antibodies with Y. pseudotuberculosis Ia and/or Y. rohdei b, d, e, f, i, which were observed by means of dot-blotting procedure using the whole bacterial cells as an antigen, is not caused by LPS of these bacteria. (en)
Title	Characterization of rabbit antibodies for immunochemical detection of Yersinia enterocolitica Characterization of rabbit antibodies for immunochemical detection of Yersinia enterocolitica (en)
skos:prefLabel	Characterization of rabbit antibodies for immunochemical detection of Yersinia enterocolitica Characterization of rabbit antibodies for immunochemical detection of Yersinia enterocolitica (en)
skos:notation	RIV/60461373:22330/07:43893202!RIV12-GA0-22330___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA525/05/0311)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Hochel, Igor
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	413350
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/07:43893202
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	lipopolysaccharides; cross-reactivity; immunoglobulins; ELISA; Yersinia enterocolitica (en)
http://linked.open.../riv/klicoveSlovo	cross-reactivity ELISA lipopolysaccharides immunoglobulins Yersinia enterocolitica
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[A466B662E164]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Rapid methods for detection of pathogenic Yersinia enterocolitica in foods
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	52
http://linked.open...iv/tvurceVysledku	Škvor, Jiří Hochel, Igor
http://linked.open...ain/vavai/riv/wos	000252006400010
issn	0015-5632
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	22330

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