About: Analysis of elements species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS     Goto   Sponge   NotDistinct   Permalink

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Description
  • Fractionation of Co, Cu, Fe, Mn, Mo, Ni, P and Zn species in extracts of buckwheat semolina and amaranth flour in 0.02 moll-1 Tris-HCl (pH=7.5) buffer was performed by SEC/ICP-MS hyphenation. Element specific chromatograms of the buckwheat extract are similar to those of legumes and cereals. Except for Fe and Mn the remaining metals are concentrated in a low-molecular fraction (Mr<2 kDa). Fe is bound in a high-molecular fraction (Mr>150 kDa). Chromatograms of Co, Fe, Mo and Ni species in amaranth extracts correspond with this pattern too. However, Cu is also bound in a medium-molecular fraction (Mr~40 kDa) and in a high-molecular one (Mr>150 kDa). The main part of Mn in both extracts is present in labile complex and ionic form. Fractions rich of trace metals were isolated using preparative-scale SEC and the organic ligands of metals were consequently preconcentrated on Chelex 100 in Cu2+ form and eluted by ammonia solution. Low content of sulphur containing amino acids (Cys, Met) and high con
  • Fractionation of Co, Cu, Fe, Mn, Mo, Ni, P and Zn species in extracts of buckwheat semolina and amaranth flour in 0.02 moll-1 Tris-HCl (pH=7.5) buffer was performed by SEC/ICP-MS hyphenation. Element specific chromatograms of the buckwheat extract are similar to those of legumes and cereals. Except for Fe and Mn the remaining metals are concentrated in a low-molecular fraction (Mr<2 kDa). Fe is bound in a high-molecular fraction (Mr>150 kDa). Chromatograms of Co, Fe, Mo and Ni species in amaranth extracts correspond with this pattern too. However, Cu is also bound in a medium-molecular fraction (Mr~40 kDa) and in a high-molecular one (Mr>150 kDa). The main part of Mn in both extracts is present in labile complex and ionic form. Fractions rich of trace metals were isolated using preparative-scale SEC and the organic ligands of metals were consequently preconcentrated on Chelex 100 in Cu2+ form and eluted by ammonia solution. Low content of sulphur containing amino acids (Cys, Met) and high con (en)
  • Frakcionace specií Co, Cu, Fe, Mn, Mo, Ni, P a Zn v extraktech pohankové krupice a amarantové mouky v 0,02 mol/l Tris-HCL (pH=7,5) byla provedena metodou SEC/ICP-MS. (cs)
Title
  • Analysis of elements species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS
  • Analýza frakcí prvkových specií v pohankové a amarantové mouce metodami SEC/ICP-MS a MALDI-MS (cs)
  • Analysis of elements species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS (en)
skos:prefLabel
  • Analysis of elements species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS
  • Analýza frakcí prvkových specií v pohankové a amarantové mouce metodami SEC/ICP-MS a MALDI-MS (cs)
  • Analysis of elements species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS (en)
skos:notation
  • RIV/60461373:22330/07:00019275!RIV08-MSM-22330___
http://linked.open.../vavai/riv/strany
  • 895-904
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika
  • 5-6
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 409461
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22330/07:00019275
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Amaranth; buckwheat; speciation; trace elements; MALDI-MS; ICP-MS (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [26AAE12DF124]
http://linked.open...i/riv/nazevZdroje
  • European Food Research and Technology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 225
http://linked.open...iv/tvurceVysledku
  • Kodíček, Milan
  • Koplík, Richard
  • Mestek, Oto
  • Polák, Jan
  • Šantrůček, Jiří
  • Kvasnička, František
  • Komínková, Jana
http://linked.open...n/vavai/riv/zamer
issn
  • 1438-2377
number of pages
http://localhost/t...ganizacniJednotka
  • 22330
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