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| Description
| - Proper identification of proteinaceous binders in artworks is essential for specification of the painting technique and thus also for selection of the restoration method; moreover, it might be helpful for the authentication of the artwork. This paper is concerned with the optimisation of analysis of the proteinaceous binders contained in the colour layers of artworks. Within this study, we worked out a method for the preparation and analysis of solid samples from artworks using tryptic cleavage and subsequent analysis of the acquired peptide mixture by matrix assisted laser desorption/ionisation time of flight mass spectrometry. To make this approach rational and efficient, we created a database of commonly used binders (egg yolk, egg white, casein, milk, curd, whey, gelatine, and various types of animal glues); certain peaks in the mass spectra of these binders, formed by rich protein mixtures, were matched to amino acid sequences of the individual proteins that were found in the Internet database Ex
- Proper identification of proteinaceous binders in artworks is essential for specification of the painting technique and thus also for selection of the restoration method; moreover, it might be helpful for the authentication of the artwork. This paper is concerned with the optimisation of analysis of the proteinaceous binders contained in the colour layers of artworks. Within this study, we worked out a method for the preparation and analysis of solid samples from artworks using tryptic cleavage and subsequent analysis of the acquired peptide mixture by matrix assisted laser desorption/ionisation time of flight mass spectrometry. To make this approach rational and efficient, we created a database of commonly used binders (egg yolk, egg white, casein, milk, curd, whey, gelatine, and various types of animal glues); certain peaks in the mass spectra of these binders, formed by rich protein mixtures, were matched to amino acid sequences of the individual proteins that were found in the Internet database Ex (en)
- Správná identifikace proteinových pojiv je nezbytná ke zjištění malířské techniky, pro zvolení vhodného postupu restaurování a také může napomoci k autentikaci díla. Článek je zaměřen na optimalizaci analysy proteinových pojiv obsažených v barevných vrstvách uměleckých děl hmotnostní spektrometrií MALDI-TOF (matrix assisted laser desorption/ionisation time of flight mass spectrometry). Pro účely identifikace pojiv byla vytvořena a vylepšena databáze referenčních vzorků proteinových pojiv. Metoda byla úspěšně otestována na modelových vzorcích podkladových vrstev a vzorcích odebraných z obrazů Edvarda Muncha. (cs)
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| Title
| - Identification of proteinaceous binders in art works by MALDI-TOF mass spectrometry
- Identification of proteinaceous binders in art works by MALDI-TOF mass spectrometry (en)
- Identifikace proteinových pojiv užívaných v uměleckých dílech pomocí MALDI-TOF hmotnostní spektrometrie (cs)
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| skos:prefLabel
| - Identification of proteinaceous binders in art works by MALDI-TOF mass spectrometry
- Identification of proteinaceous binders in art works by MALDI-TOF mass spectrometry (en)
- Identifikace proteinových pojiv užívaných v uměleckých dílech pomocí MALDI-TOF hmotnostní spektrometrie (cs)
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| skos:notation
| - RIV/60461373:22330/07:00019041!RIV08-MSM-22330___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/07:00019041
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - proteinaceous binders; MALDI-TOF; Edvard Munch (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Analytical and Bioanalytical Chemistry
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Hynek, Radovan
- Kodíček, Milan
- Kučková, Štěpánka
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| http://linked.open...n/vavai/riv/zamer
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| http://localhost/t...ganizacniJednotka
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