About: Radioimmunoassay of 16α-Hydroxy-Dehydroepiandrosterone and its Physiological levels

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About: Radioimmunoassay of 16α-Hydroxy-Dehydroepiandrosterone and its Physiological levels Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Práce popisuje vývoj imunoanalytické metody pro 16α-Hydroxy-dehydroepiandrosteron (cs) 16α-Hydroxy-dehydroepiandrosterone (16α-OH-DHEA) belongs to the products of extensive DHEA metabolism in mammalian tissues. It is a precursor of 16α-hydroxylated estrogens, increased levels of which are associated with autoimmune disorders. A highly specific radioimmunoassay of unconjugated 16α-OH-DHEA was developed and evaluated. Polyclonal rabbit antisera were raised against 3α,16α- dihydroxy-17,19-dione-19-O-(carboxymethyloxime) and 3α,16α-dihydroxy-7,17-dione-7-O-(carboxymethyloxime) BSA conjugates. Two methods were used for preparation of the conjugates. Homologous radioiodinated derivatives with tyrosine methyl ester were prepared as tracers. While antisera to 7-CMO cross-reacted with DHEA as much as by 58%, the cross-reaction of the chosen antiserum prepared via 19-oxogroup by micellar conjugation technique with 16α-OH-DHEA was only 0.13% and with all other structurally related steroids, including DHEA were lower than 0.01%. The detection limi 16α-Hydroxy-dehydroepiandrosterone (16α-OH-DHEA) belongs to the products of extensive DHEA metabolism in mammalian tissues. It is a precursor of 16α-hydroxylated estrogens, increased levels of which are associated with autoimmune disorders. A highly specific radioimmunoassay of unconjugated 16α-OH-DHEA was developed and evaluated. Polyclonal rabbit antisera were raised against 3α,16α- dihydroxy-17,19-dione-19-O-(carboxymethyloxime) and 3α,16α-dihydroxy-7,17-dione-7-O-(carboxymethyloxime) BSA conjugates. Two methods were used for preparation of the conjugates. Homologous radioiodinated derivatives with tyrosine methyl ester were prepared as tracers. While antisera to 7-CMO cross-reacted with DHEA as much as by 58%, the cross-reaction of the chosen antiserum prepared via 19-oxogroup by micellar conjugation technique with 16α-OH-DHEA was only 0.13% and with all other structurally related steroids, including DHEA were lower than 0.01%. The detection limi (en)
Title	Radioimmunoassay of 16α-Hydroxy-Dehydroepiandrosterone and its Physiological levels Radioimmunoassay of 16α-Hydroxy-Dehydroepiandrosterone and its Physiological levels (en) Radioimmunoassay of 16α-Hydroxy-Dehydroepiandrosteronu a jeho fyziologické hladiny (cs)
skos:prefLabel	Radioimmunoassay of 16α-Hydroxy-Dehydroepiandrosterone and its Physiological levels Radioimmunoassay of 16α-Hydroxy-Dehydroepiandrosterone and its Physiological levels (en) Radioimmunoassay of 16α-Hydroxy-Dehydroepiandrosteronu a jeho fyziologické hladiny (cs)
skos:notation	RIV/60461373:22330/07:00018983!RIV08-MSM-22330___
http://linked.open.../vavai/riv/strany	130-135
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Lapčík, Oldřich
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	446319
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/07:00018983
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	16α-Hydroxy-dehydroepiandrosterone; DHEA; RIA; immunoassay; steroid (en)
http://linked.open.../riv/klicoveSlovo	RIA steroid DHEA 16α-Hydroxy-dehydroepiandrosterone immunoassay
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[0AA943207192]
http://linked.open...i/riv/nazevZdroje	Journal of Steroid Biochemistry and Molecular Biology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	104
http://linked.open...iv/tvurceVysledku	Hampl, Richard Hill, Martin Lapčík, Oldřich Kazihnitková, Hana Zamrazilová, Ludmila
http://linked.open...n/vavai/riv/zamer	Teoretické základy potravinářských a biochemických technologií
issn	0960-0760
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	22330

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