About: Biotransformation of nitriles by Rhodococcus equi A4 immobilized in LentiKats(R).     Goto   Sponge   NotDistinct   Permalink

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Description
  • Whole cells of Rhodococcus equi A4, a producer of nitrile hydratase and amidase activities, were immobilized in lens-shaped hydrogel particles, LentiKats(R). The immobilized biocatalyst was applied to the biotransformation of benzonitrile, 3-cyanopyridine, (R,S)-3-hydroxy-2-methylene-butanenitrile and (R,S)-3-hydroxy-2-methylene-3-phenyl-propanenitrile. The stability of the nitrile hydratase during the repeated use of the biocatalyst was dependent on the type of the substrate. The enzyme was the most stable during the transformation of (R,S)-3-hydroxy-2-methylene-butanenitrile. No significant loss of the amidase activity was observed within the biocatalyst operation
  • Whole cells of Rhodococcus equi A4, a producer of nitrile hydratase and amidase activities, were immobilized in lens-shaped hydrogel particles, LentiKats(R). The immobilized biocatalyst was applied to the biotransformation of benzonitrile, 3-cyanopyridine, (R,S)-3-hydroxy-2-methylene-butanenitrile and (R,S)-3-hydroxy-2-methylene-3-phenyl-propanenitrile. The stability of the nitrile hydratase during the repeated use of the biocatalyst was dependent on the type of the substrate. The enzyme was the most stable during the transformation of (R,S)-3-hydroxy-2-methylene-butanenitrile. No significant loss of the amidase activity was observed within the biocatalyst operation (en)
  • Rhodococcus equi A4, producent nitrilhydratasové a amidasové aktivity byl imobilizován v lentiketových hydrogelových partikulích LentiKats(R). Imobilizovaný biokatalyzátor byl aplikován v biotransformaci benzonitrilu, 3-cyanopyridinu, (R,S)-3-hydroxy-2-methylene-butanenitrilu and (R,S)-3-hydroxy-2-methylene-3-phenyl-propanenitrilu. Stabilita nitrilhydratasy během opakovaného použití biokatalyzátoru byla závislá na typu substrátu.Během použití nebyla zaznamenána výrazná aktivita amidasy. (cs)
Title
  • Biotransformation of nitriles by Rhodococcus equi A4 immobilized in LentiKats(R).
  • Biotransformation of nitriles by Rhodococcus equi A4 immobilized in LentiKats(R). (en)
  • Biotransformace nitrilů Rhodococcus equi A4 imobilizovaných v LentiKats(R). (cs)
skos:prefLabel
  • Biotransformation of nitriles by Rhodococcus equi A4 immobilized in LentiKats(R).
  • Biotransformation of nitriles by Rhodococcus equi A4 immobilized in LentiKats(R). (en)
  • Biotransformace nitrilů Rhodococcus equi A4 imobilizovaných v LentiKats(R). (cs)
skos:notation
  • RIV/60461373:22330/06:00017788!RIV07-MSM-22330___
http://linked.open.../vavai/riv/strany
  • 59-61
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika
  • 39
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 467149
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22330/06:00017788
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Nitrile hydratase; amidase; nitriles; Rhodococcus equi; LentiKats(R) (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [56DEE4F78FFE]
http://linked.open...i/riv/nazevZdroje
  • Journal of Molecular Catalysis B
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Martínková, Ludmila
  • Stloukal, Radek
  • Masák, Jan
  • Jirků, Vladimír
  • Čejková, Alena
  • Kubáč, David
  • Galliene, Estelle
  • Lemaire, Marielle
http://linked.open...n/vavai/riv/zamer
issn
  • 1381-1177
number of pages
http://localhost/t...ganizacniJednotka
  • 22330
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