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  • Two methods of preservation and storage of yeast cultures are tested. Freest yeast population suspended at medium containing glycerol (5% v/v) as a cryoprotectant and periodic sub-culture using agar slopes (slants). This work was carried out with three widely used Czech lager strains - No. 2, 7 and 95. All yeasts were analyzed by flow cytometry prior to storage and after storage. Fluorescent intensity of DNA was analysed by flow cytometry and signal was used as a marker for cell cycle determination. During experiments were monitored changes in significant properties of yeast (glycogen, proteins, DNA) by fluorescent methods. It seems that the impact of storage conditions has not influence on basic characteristic of yeast population, dynamic of microbial growth, cell cycle and technological properties of yeast cultures.
  • Two methods of preservation and storage of yeast cultures are tested. Freest yeast population suspended at medium containing glycerol (5% v/v) as a cryoprotectant and periodic sub-culture using agar slopes (slants). This work was carried out with three widely used Czech lager strains - No. 2, 7 and 95. All yeasts were analyzed by flow cytometry prior to storage and after storage. Fluorescent intensity of DNA was analysed by flow cytometry and signal was used as a marker for cell cycle determination. During experiments were monitored changes in significant properties of yeast (glycogen, proteins, DNA) by fluorescent methods. It seems that the impact of storage conditions has not influence on basic characteristic of yeast population, dynamic of microbial growth, cell cycle and technological properties of yeast cultures. (en)
  • V tomto příspěvku jsou porovnány dvě metody uchovávání laboratorních a průmyslových kmenů kvasinky Saccharomyces cerevisiae za použití průtokové cytometrie.Metody jsou zhodnoceny po stránce praktické a ekonomické náročnosti a dostupnosti. (cs)
Title
  • Flow cytometric analysis of Saccharomyces cerevisiae
  • Flow cytometric analysis of Saccharomyces cerevisiae (en)
  • Analýza Saccharomyces cerevisiae průtokovou cytometrií (cs)
skos:prefLabel
  • Flow cytometric analysis of Saccharomyces cerevisiae
  • Flow cytometric analysis of Saccharomyces cerevisiae (en)
  • Analýza Saccharomyces cerevisiae průtokovou cytometrií (cs)
skos:notation
  • RIV/60461373:22330/06:00017747!RIV07-MSM-22330___
http://linked.open...avai/riv/aktivita
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  • P(1M0570), Z(MSM6046137305)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 476026
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  • RIV/60461373:22330/06:00017747
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  • flow cytometry; Saccharomyces cerevisiae; beer; storage (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...i/riv/kodPristupu
http://linked.open...ontrolniKodProRIV
  • [611E294B626A]
http://linked.open...i/riv/mistoVydani
  • Zoeterwoude, The Netherlands
http://linked.open...n/vavai/riv/nosic
  • 2006
http://linked.open...telVyzkumneZpravy
  • European Brewery Convention
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Fiala, Jaromír
  • Slabý, Martin
http://linked.open...n/vavai/riv/zamer
http://localhost/t...ganizacniJednotka
  • 22330
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