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Description
  • Yeast activity was monitored during pilot plant yeast propagation under aerobic and anaerobic conditions. The work was carried out with three widely used Czech lager yeast strains. Fluorescent intensity of DNA was analyzed by flow cytometry and signal was used as a marker for cell cycle determination. During experiments were monitored changes in significant properties of yeast (glycogen, proteins, and DNA) by fluorescent methods. It seemed that the impact of aerobic conditions on preservation of flocculation abilities depends on the genetic properties of each strain. Only one strain didn't show significant losses in flocculation abilities neither under aerobic nor strictly anaerobic conditions. (en)
  • Yeast activity was monitored during pilot plant yeast propagation under aerobic and anaerobic conditions. The work was carried out with three widely used Czech lager yeast strains. Fluorescent intensity of DNA was analyzed by flow cytometry and signal was used as a marker for cell cycle determination. During experiments were monitored changes in significant properties of yeast (glycogen, proteins, and DNA) by fluorescent methods. It seemed that the impact of aerobic conditions on preservation of flocculation abilities depends on the genetic properties of each strain. Only one strain didn't show significant losses in flocculation abilities neither under aerobic nor strictly anaerobic conditions.
  • Aktivita kvasinek byla sledována v průběhu poloprovozní propagace kvasnic v aerobních a anaerobních podmínkách. Byly použity tři kmeny spodně kvasících kvasnic, běžně používané v českých pivovarech. Fluorescenční intenzita DNA byla analyzována průtokovou cytometrií, a signál byl použit jako marker k určení buněčného cyklu. V průběhu experimentu byly sledovány změny významných vlastností kvasinek (obsah glykogenu, proteinů, DNA) fluorescenční metodou. Zdá se, že vliv aerobních podmínek na zachování flokulačních schopností závisí na genetických vlastnostech každého kmene. Pouze jeden kmen nevykázal významné ztráty flokulačních schopností jak v aerobních, tak v striktně anaerobních podmínkách. (cs)
Title
  • Yeast activity monitoring by fluorescent methods-sborník
  • Yeast activity monitoring by fluorescent methods-sborník (en)
  • Sledování aktivity kvasinek fluorescenční metodou (cs)
skos:prefLabel
  • Yeast activity monitoring by fluorescent methods-sborník
  • Yeast activity monitoring by fluorescent methods-sborník (en)
  • Sledování aktivity kvasinek fluorescenční metodou (cs)
skos:notation
  • RIV/60461373:22330/05:00016246!RIV06-MSM-22330___
http://linked.open.../vavai/riv/strany
  • 368-375
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1M0570)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 551615
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22330/05:00016246
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Aeration; cell cycle; flow cytometry; fluorescent methods; yeast physiology; yeast propagation (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [5C51CAD1A2A5]
http://linked.open...v/mistoKonaniAkce
  • Praha
http://linked.open...i/riv/mistoVydani
  • Norimberk
http://linked.open...i/riv/nazevZdroje
  • Proceedings of the 30th EBC Congress - CD-ROM
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Dostálek, Pavel
  • Novák, Jan
  • Fiala, Jaromír
  • Basařová, Gabriela
  • Teixeira, José
  • Vicente, António
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
http://purl.org/ne...btex#hasPublisher
  • Fachverlag Hans Carl
https://schema.org/isbn
  • 90-70143-23-2
http://localhost/t...ganizacniJednotka
  • 22330
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