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  • Molecular biology assays were used to determine the identification of thermophilic and mesophilic bacilli. A genotypic study using randomly amplified polymorphic DNA analysis (RAPD), amplification of the 16S-23S rRNA intergenic transcribed spacer (ITS-PCR), restriction fragment length polymorphism (RFLP) of 16S rRNA and restriction fragment length polymorphism of the ITS-PCR (ITS-PCR RFLP) was carried out with 5 thermophilic and 3 mesophilic Bacillus isolates. Protocols for all above mentioned methods were optimised for routine laboratory use.
  • Molecular biology assays were used to determine the identification of thermophilic and mesophilic bacilli. A genotypic study using randomly amplified polymorphic DNA analysis (RAPD), amplification of the 16S-23S rRNA intergenic transcribed spacer (ITS-PCR), restriction fragment length polymorphism (RFLP) of 16S rRNA and restriction fragment length polymorphism of the ITS-PCR (ITS-PCR RFLP) was carried out with 5 thermophilic and 3 mesophilic Bacillus isolates. Protocols for all above mentioned methods were optimised for routine laboratory use. (en)
  • Metody molekulární biologie byla použity pro identifikaci termofilních a mezofilních bakterií rodu Bacillus. Bylo zkoumáno celkem 5 termofilních a 3 mezofilní izoláty a to pomocí metod: polymorfismus náhodně zmnožené DNA (RAPD), amplifikace intergenové oblasti 16S-23S rRNA (ITS-PCR) a polymorfie délky restrikčních fragmentů (RFLP) jak 16S rRNA tak produktů ITS-PCR reakce. Pro všechny výše uvedené metody byly optimalizovány jejich protokoly pro snadné laboratorní použití. (cs)
Title
  • Molecular characterization of thermophilic and mesophilic Bacillus species
  • Charakterizace termofilních a mezofilních druhů rodu Bacillus (cs)
  • Molecular characterization of thermophilic and mesophilic Bacillus species (en)
skos:prefLabel
  • Molecular characterization of thermophilic and mesophilic Bacillus species
  • Charakterizace termofilních a mezofilních druhů rodu Bacillus (cs)
  • Molecular characterization of thermophilic and mesophilic Bacillus species (en)
skos:notation
  • RIV/60461373:22330/05:00015945!RIV06-GA0-22330___
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  • P(GA525/03/0375)
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  • 530999
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  • RIV/60461373:22330/05:00015945
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  • thermophilic Bacillus; mesophilic Bacillus; PCR; RAPD; RFLP; ITS-PCR; ITS-PCR RFLP (en)
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  • [9F98B5B39746]
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  • Melzoch, Karel
  • Patáková, Petra
  • Piterková, Lucie
  • Skarkeová, Martina
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  • 22330
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