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rdf:type
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Description
| - Two variants of immunoassay for determination of biochanin A (5,7-dihydroxy 4´-methoxy isoflavone), i.e. the RIA and the indirect ELISA, have been developed and evaluated. Both methods employ the same rabbit antiserum to a 7-O-carboxymethyl-5-hydroxy-4'-methoxyisoflavone # bovine serum albumin conjugate. A 125I labeled hapten-tyrosine methyl ester conjugate was used as radioligand for the RIA. The indirect ELISA uses immunogen coated microtitration plates and a peroxidase labeled anti-rabbit Ig antibody.Both methods are specific for biochanin A with a comparable sensitivity (3.1 pg/tube for RIA; 5.3 pg/well for ELISA), however their sensitivity to individual crossreactants differs. Main crossreactants are sissotrin (the crossreactivity 15.7% for RIA; 120% for ELISA), 5-hydroxy, 4´,7-dimethoxy isoflavone (51.5%, RIA; 46.5%, ELISA), prunetin (4.5%, RIA; 5.0%, ELISA), genistein (0.8%, RIA; 2.8%, ELISA) and formononetin (0.4%, RIA; 0.3%, ELISA). The method was used for analysis of biochanin A in alfa
- Two variants of immunoassay for determination of biochanin A (5,7-dihydroxy 4´-methoxy isoflavone), i.e. the RIA and the indirect ELISA, have been developed and evaluated. Both methods employ the same rabbit antiserum to a 7-O-carboxymethyl-5-hydroxy-4'-methoxyisoflavone # bovine serum albumin conjugate. A 125I labeled hapten-tyrosine methyl ester conjugate was used as radioligand for the RIA. The indirect ELISA uses immunogen coated microtitration plates and a peroxidase labeled anti-rabbit Ig antibody.Both methods are specific for biochanin A with a comparable sensitivity (3.1 pg/tube for RIA; 5.3 pg/well for ELISA), however their sensitivity to individual crossreactants differs. Main crossreactants are sissotrin (the crossreactivity 15.7% for RIA; 120% for ELISA), 5-hydroxy, 4´,7-dimethoxy isoflavone (51.5%, RIA; 46.5%, ELISA), prunetin (4.5%, RIA; 5.0%, ELISA), genistein (0.8%, RIA; 2.8%, ELISA) and formononetin (0.4%, RIA; 0.3%, ELISA). The method was used for analysis of biochanin A in alfa (en)
- ELISA a RIA metody pro stanovení biochaninu A byly vyvinuty na základě téhož polyklonálního králičího antiséra proti konjugátu biochaninu s hovězím sérovým albuminem. Obě metody jsou srovnatelně specifické a citlivé pro biochanin A (3.1 pg/zkumavku pro RIA; 5.3 pg/jamku pro ELISA), ale liší se v citlivosti vůči některým křížovým reaktantům. Metody byly použity pro stanovení biochaninu A ve vojtěšce a v několika ne-bobovitých rostlinách. (cs)
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Title
| - Immunoassay for biochanin A
- Immunoassay for biochanin A (en)
- Imunoanalýza biochaninu A (cs)
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skos:prefLabel
| - Immunoassay for biochanin A
- Immunoassay for biochanin A (en)
- Imunoanalýza biochaninu A (cs)
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skos:notation
| - RIV/60461373:22330/04:00012802!RIV/2005/GA0/223305/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA525/03/0352), Z(MSM 223300004)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/04:00012802
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Isoflavone;biochanin A;RIA;ELISA;immunoassay (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Journal of Immunological Methods
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Lapčík, Oldřich
- Klejdus, Bořivoj
- Vítková, Michaela
- Al-Maharik, N.
- Adlercreutz, H.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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