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rdf:type
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Description
| - funkce proteasových domén MPMV (cs)
- The role of retroviral proteases is well known. These aspartic proteases are active as homodimers and cleave Gag polyprotein precursors during particle release. M-PMV assembles immature particles in the cytoplasm and activation of the protease within the immature particles is delayed until viral budding. Gene encoding M-PMV PR is located at the 3' end of the pro ORF. The first active form of the protease is a 17 kDa protein, containing about 50 amino acids C-terminal domain which is characteristic only for betaretroviruses. Other structural elements and the overall structure of M-PMC PR are similar to other retroviral proteases1. The 17 kDa PR undergoes an autocatalytic processing from the C-terminus yielding 13 and 12 kDa proteases2. Here we have investigated the role of C-terminal domain of PR for the maturation of M-PMV. Sequences encoding the C-terminal domain of PR were deleted in the M-PMV genome and the processing of Gag polyproteins, the RT activity, and viral infectivity were analysed. T
- The role of retroviral proteases is well known. These aspartic proteases are active as homodimers and cleave Gag polyprotein precursors during particle release. M-PMV assembles immature particles in the cytoplasm and activation of the protease within the immature particles is delayed until viral budding. Gene encoding M-PMV PR is located at the 3' end of the pro ORF. The first active form of the protease is a 17 kDa protein, containing about 50 amino acids C-terminal domain which is characteristic only for betaretroviruses. Other structural elements and the overall structure of M-PMC PR are similar to other retroviral proteases1. The 17 kDa PR undergoes an autocatalytic processing from the C-terminus yielding 13 and 12 kDa proteases2. Here we have investigated the role of C-terminal domain of PR for the maturation of M-PMV. Sequences encoding the C-terminal domain of PR were deleted in the M-PMV genome and the processing of Gag polyproteins, the RT activity, and viral infectivity were analysed. T (en)
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Title
| - FUNCTIONS OF PROTEASE DOMAINS IN M-PMV
- FUNCTIONS OF PROTEASE DOMAINS IN M-PMV (en)
- funkce proteasových domén MPMV (cs)
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skos:prefLabel
| - FUNCTIONS OF PROTEASE DOMAINS IN M-PMV
- FUNCTIONS OF PROTEASE DOMAINS IN M-PMV (en)
- funkce proteasových domén MPMV (cs)
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skos:notation
| - RIV/60461373:22330/04:00012789!RIV/2005/MSM/223305/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/04:00012789
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
| - The retrovirus assembly meeting
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Pichová, Iva
- Ruml, Tomáš
- Hunter, Eric
- Bauerová, Helena
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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number of pages
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http://purl.org/ne...btex#hasPublisher
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https://schema.org/isbn
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http://localhost/t...ganizacniJednotka
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