About: In vitro distribution and characterisation of membrane associated PLD and PI-PLC in Brassica napus     Goto   Sponge   NotDistinct   Permalink

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  • Two types of phospholipid degrading enzymes phospholipase D (PLD; EC 3.1.4.4) and phosphatidyl inositol specific phospholipase C (PIP2-PLC; PI-PLC 3.1.4.11) were studied during the development of seeds and plants of Brassica napus. PLD exhibits two typesof activities; polyphosphoinositide requiring (PIP2-dependent PLD) and polyphosphoinositide independent requiring millimolar concentration of calcium (PLD?). Significantly different patterns of the activity profiles were found for soluble and membrane a ssociated forms of all three enzymes within both processes. Membrane associated PIP2-dependent PLD activity shows the opposite trend when compared to PLD?, while highest PI-PLC activity appears in the same stages of development of seeds and plants as forPLD?. In subcellular fractions of hypocotyls parts of young plants phospholipases were localized predominantly on plasma membranes. Biochemical characteristics (Ca2+, pH) of all three enzymes associated with plasma membrane vesicles isolated by partitio
  • Two types of phospholipid degrading enzymes phospholipase D (PLD; EC 3.1.4.4) and phosphatidyl inositol specific phospholipase C (PIP2-PLC; PI-PLC 3.1.4.11) were studied during the development of seeds and plants of Brassica napus. PLD exhibits two typesof activities; polyphosphoinositide requiring (PIP2-dependent PLD) and polyphosphoinositide independent requiring millimolar concentration of calcium (PLD?). Significantly different patterns of the activity profiles were found for soluble and membrane a ssociated forms of all three enzymes within both processes. Membrane associated PIP2-dependent PLD activity shows the opposite trend when compared to PLD?, while highest PI-PLC activity appears in the same stages of development of seeds and plants as forPLD?. In subcellular fractions of hypocotyls parts of young plants phospholipases were localized predominantly on plasma membranes. Biochemical characteristics (Ca2+, pH) of all three enzymes associated with plasma membrane vesicles isolated by partitio (en)
Title
  • In vitro distribution and characterisation of membrane associated PLD and PI-PLC in Brassica napus
  • In vitro distribution and characterisation of membrane associated PLD and PI-PLC in Brassica napus (en)
skos:prefLabel
  • In vitro distribution and characterisation of membrane associated PLD and PI-PLC in Brassica napus
  • In vitro distribution and characterisation of membrane associated PLD and PI-PLC in Brassica napus (en)
skos:notation
  • RIV/60461373:22330/03:00008957!RIV/2004/GA0/223304/N
http://linked.open.../vavai/riv/strany
  • 691-698
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA522/00/1332)
http://linked.open...iv/cisloPeriodika
  • 54
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 610057
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22330/03:00008957
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • phospholipases; Brassica napus; plasma membrane (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [A075A2B83D89]
http://linked.open...i/riv/nazevZdroje
  • Journal of Experimental Botany
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 2003
http://linked.open...iv/tvurceVysledku
  • Martinec, Jan
  • Novotná, Zuzana
  • Valentová, Olga
  • Profotová, Bronislava
  • Kader, Jean-Claude
  • Žďárová, Štěpánka
issn
  • 0022-0957
number of pages
http://localhost/t...ganizacniJednotka
  • 22330
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