About: Development of an indirect competitive enzyme-linked immunosorbent assay of DDT

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Indirect competitive enzyme immunoassays of DDT based on polyclonal antibodies have been developed. The limit of detection and limit of quantification were 0.3 nmol l-1 and 4.2 nmol l-1, respectively. Non-specific interaction was eliminated by the saturation of the solid phase with 2 % human serum albumin. Different polystyrene microplates were tested. The cross-reactivity of the polyclonal rabbit antibody to DDT with 2,4'-DDE, 4,4'-DDD, 2,4'-DDT, 2,4'-DDD, Methoxychlor and 4,4'-DDE was 1 %, 9 %, 12 %, 13 %, 36 % and 186 %, respectively. No cross-reactivity of the tested antibody with 4,4'-DDA, 4,4'-DDOH, 4,4'-DBP, 4,4'-DDM, 4,4'-PCB, 2,3'-PCB, 4-PCB and 3-PCB was observed. With respect to a limited solubility of the analyte in water, the influence of different concentrations of methanol, ethanol, acetone and N,N'-dimethylformamide on the assay sensitivity were tested. A recommended concentration of the organic solvents is 5-10 % (v/v) in the reaction mixture. Preliminary tests were done with a limite Indirect competitive enzyme immunoassays of DDT based on polyclonal antibodies have been developed. The limit of detection and limit of quantification were 0.3 nmol l-1 and 4.2 nmol l-1, respectively. Non-specific interaction was eliminated by the saturation of the solid phase with 2 % human serum albumin. Different polystyrene microplates were tested. The cross-reactivity of the polyclonal rabbit antibody to DDT with 2,4'-DDE, 4,4'-DDD, 2,4'-DDT, 2,4'-DDD, Methoxychlor and 4,4'-DDE was 1 %, 9 %, 12 %, 13 %, 36 % and 186 %, respectively. No cross-reactivity of the tested antibody with 4,4'-DDA, 4,4'-DDOH, 4,4'-DBP, 4,4'-DDM, 4,4'-PCB, 2,3'-PCB, 4-PCB and 3-PCB was observed. With respect to a limited solubility of the analyte in water, the influence of different concentrations of methanol, ethanol, acetone and N,N'-dimethylformamide on the assay sensitivity were tested. A recommended concentration of the organic solvents is 5-10 % (v/v) in the reaction mixture. Preliminary tests were done with a limite (en)
Title	Development of an indirect competitive enzyme-linked immunosorbent assay of DDT Development of an indirect competitive enzyme-linked immunosorbent assay of DDT (en)
skos:prefLabel	Development of an indirect competitive enzyme-linked immunosorbent assay of DDT Development of an indirect competitive enzyme-linked immunosorbent assay of DDT (en)
skos:notation	RIV/60461373:22330/03:00008938!RIV/2004/GA0/223304/N
http://linked.open.../vavai/riv/strany	285-300
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA525/02/0287)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Hochel, Igor Musil, Marek
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	603469
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/03:00008938
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ELISA, DDT, cross-reactivity, liquid-liquid extraction, matrix effect (en)
http://linked.open.../riv/klicoveSlovo	cross-reactivity liquid-liquid extraction DDT ELISA matrix effect
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[A752A11E08E9]
http://linked.open...i/riv/nazevZdroje	Food and Agricultural Immunology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of screening ELISA and PCR methods for detection of Campylobacteria in foods
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	14
http://linked.open...iv/tvurceVysledku	Musil, Marek Hochel, Igor
issn	0954-0105
number of pages	16 (xsd:int)
http://localhost/t...ganizacniJednotka	22330

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