About: Optimisation of enzyme-linked immunosorbent assay for the detection of Listeria monocytogenes

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About: Optimisation of enzyme-linked immunosorbent assay for the detection of Listeria monocytogenes Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Listeria monocytogenes is a gram-positive rod-shaped bacterium associated with a variety of environments including soils, water, sewage, silage, as well as plant and animal food products. Although reported cases of human foodborne listeriosis are rare, the incidence of serious illness and death in affected individuals is high. The detection of Listeria monocytogenes cells by conventional microbiological methods takes 5-6 days. We present here optimizations of a rapid, reliable and specific antibody-based assay. A model system consists of an enrichment stage and end-point detection by enzyme linked immunosorbent assay. Listeria cells were cultivated in selective medium in first step. Enrichment stage was followed by direct sandwich format of ELISA. It was found both convenient culture medium for cultivation of bacterial cells for use in the ELISA and optimal buffers for a whole method. From all tested antibodies, which have been raised, was found as the best that one against Listeria monocytogen Listeria monocytogenes is a gram-positive rod-shaped bacterium associated with a variety of environments including soils, water, sewage, silage, as well as plant and animal food products. Although reported cases of human foodborne listeriosis are rare, the incidence of serious illness and death in affected individuals is high. The detection of Listeria monocytogenes cells by conventional microbiological methods takes 5-6 days. We present here optimizations of a rapid, reliable and specific antibody-based assay. A model system consists of an enrichment stage and end-point detection by enzyme linked immunosorbent assay. Listeria cells were cultivated in selective medium in first step. Enrichment stage was followed by direct sandwich format of ELISA. It was found both convenient culture medium for cultivation of bacterial cells for use in the ELISA and optimal buffers for a whole method. From all tested antibodies, which have been raised, was found as the best that one against Listeria monocytogen (en)
Title	Optimisation of enzyme-linked immunosorbent assay for the detection of Listeria monocytogenes Optimisation of enzyme-linked immunosorbent assay for the detection of Listeria monocytogenes (en)
skos:prefLabel	Optimisation of enzyme-linked immunosorbent assay for the detection of Listeria monocytogenes Optimisation of enzyme-linked immunosorbent assay for the detection of Listeria monocytogenes (en)
skos:notation	RIV/60461373:22330/03:00008921!RIV/2004/GA0/223304/N
http://linked.open.../vavai/riv/strany	39
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA525/03/0350)
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Karasová, Ludmila Blažková, Martina Rauch, Pavel
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	619839
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/03:00008921
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ELISA; Listeria monocytogenes;internalin B (en)
http://linked.open.../riv/klicoveSlovo	ELISA Listeria monocytogenes internalin B
http://linked.open...ontrolniKodProRIV	[95394E0357A3]
http://linked.open...v/mistoKonaniAkce	CZ
http://linked.open...i/riv/mistoVydani	FIRMA-JK
http://linked.open...i/riv/nazevZdroje	1st International Symposium on Recent Advances in Food Analysis, Book of Abstracts
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of screening immunochemical method for specific detection of Listeria monocytogenes in foods
http://linked.open...UplatneniVysledku	2003
http://linked.open...iv/tvurceVysledku	Blažková, Martina Rauch, Pavel Karamonová, Ludmila Wyatt, Gary M.
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2003-11-05 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Vysoká škola chemicko-technologická v Praze
https://schema.org/isbn	80-7080-528-5
http://localhost/t...ganizacniJednotka	22330

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