About: Methyltransferase RcpSBI from Rhodobacter capsulatus SB1003.

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About: Methyltransferase RcpSBI from Rhodobacter capsulatus SB1003. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Rhodobacter capsulatus belongs to group of purple nonsulfur bacteria. During the sequencing project Rhodobacter capsulatus SB1003 complete nucleotide sequence of its 3.7 Mbp chromosome and 133 kbp plasmid was determined. Three different clusters of genesencoding type I (RcpSBI, RcpSBII) and type III (RcpSBIII) DNA restriction and modification systems were found. Here we describe genetic and biochemical analysis of methyltransferase (MTase) RcpSBI. It comprises three subunits: one DNA sequence specifici ty subunit (HsdS) and two DNA modification subunits (HsdM). Expression vectors carrying hsdS and hsdM genes encoding MTase activity of RcpSBI were constructed. Both genes were expressed together in E. coli and complex of 44.2 kDa HsdS and 54.3 kDa HsdM was isolated by using affinity chromatography. The isoelectric point was determined to be 5.9. The HsdS:HsdM stechiometry of MTase complex was verified as 1:2. The recognition sequence was determined as AGAN7RTAG using radioactive substrate (SAM) and dif Rhodobacter capsulatus belongs to group of purple nonsulfur bacteria. During the sequencing project Rhodobacter capsulatus SB1003 complete nucleotide sequence of its 3.7 Mbp chromosome and 133 kbp plasmid was determined. Three different clusters of genesencoding type I (RcpSBI, RcpSBII) and type III (RcpSBIII) DNA restriction and modification systems were found. Here we describe genetic and biochemical analysis of methyltransferase (MTase) RcpSBI. It comprises three subunits: one DNA sequence specifici ty subunit (HsdS) and two DNA modification subunits (HsdM). Expression vectors carrying hsdS and hsdM genes encoding MTase activity of RcpSBI were constructed. Both genes were expressed together in E. coli and complex of 44.2 kDa HsdS and 54.3 kDa HsdM was isolated by using affinity chromatography. The isoelectric point was determined to be 5.9. The HsdS:HsdM stechiometry of MTase complex was verified as 1:2. The recognition sequence was determined as AGAN7RTAG using radioactive substrate (SAM) and dif (en)
Title	Methyltransferase RcpSBI from Rhodobacter capsulatus SB1003. Methyltransferase RcpSBI from Rhodobacter capsulatus SB1003. (en)
skos:prefLabel	Methyltransferase RcpSBI from Rhodobacter capsulatus SB1003. Methyltransferase RcpSBI from Rhodobacter capsulatus SB1003. (en)
skos:notation	RIV/60461373:22330/03:00008529!RIV/2004/MSM/223304/N
http://linked.open.../vavai/riv/strany	275
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(LN00A079)
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Jenčová, Věra Strnad, Hynek Pačes, Václav Ulbrich, Pavel
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	615105
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/03:00008529
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Methyltransferase RepSBI, Rhodobacter capsulatus, purple nonsulfur bacteria (en)
http://linked.open.../riv/klicoveSlovo	Methyltransferase RepSBI Rhodobacter capsulatus purple nonsulfur bacteria
http://linked.open...ontrolniKodProRIV	[0C1CD438D804]
http://linked.open...v/mistoKonaniAkce	Drážďany
http://linked.open...i/riv/mistoVydani	Frankfurt/Main
http://linked.open...i/riv/nazevZdroje	ELSO 2003 Proceedings
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of Integrated Genomics
http://linked.open...UplatneniVysledku	2003
http://linked.open...iv/tvurceVysledku	Pačes, Václav Strnad, Hynek Ulbrich, Pavel Jenčová, Věra
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2003-09-20 (xsd:date)
issn	0021-9525
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	GBM
http://localhost/t...ganizacniJednotka	22330

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