About: Polyclonal antibodies for detection of flour mite Acarus siro

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About: Polyclonal antibodies for detection of flour mite Acarus siro Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Acaroid mites are serious pests of stored products and households. They produce allergens and transmit mycotoxin-producing fungi. The infestation of stored food by mites strongly decreases food safety and endangers public health. There is urgent need for Acaroid mites are serious pests of stored products and households. They produce allergens and transmit mycotoxin-producing fungi. The infestation of stored food by mites strongly decreases food safety and endangers public health. There is urgent need for (en) Acaroid mites are serious pests of stored products and households. They produce allergens and transmit mycotoxin-producing fungi. The infestation of stored food by mites strongly decreases food safety and endangers public health. There is urgent need forrapid detection methods such as ELISA. The aim of our work was therefore to prepare and characterise antibodies for detection of the flour mite (Acarus siro). Two polyclonal antibodies (anti- A.siroK53 and anti-A.siroK54) were prepared against an antigen in the form of protein fraction from whole body homogenates of Acarus siro. Specificity and sensitivity of the prepared antibodies were evaluated. We did not observe any cross-reactivity with stored beetles, moths, other mite species, micro-fungi, therearing diet of mites (yeast and wheat diet) and extracts from wheat kernels. Cross-reactivity to Acarus gracilis was found out. This phenomenon is discussed in relation to the evolution position of this species. The obtained antibodies (anti-A.siroK53 a (cs)
Title	Polyclonal antibodies pro detekci moucného cerva Acarus siro (cs) Polyclonal antibodies for detection of flour mite Acarus siro Polyclonal antibodies for detection of flour mite Acarus siro (en)
skos:prefLabel	Polyclonal antibodies pro detekci moucného cerva Acarus siro (cs) Polyclonal antibodies for detection of flour mite Acarus siro Polyclonal antibodies for detection of flour mite Acarus siro (en)
skos:notation	RIV/60460709:_____/04:01:_3621!RIV06-MZE-60460709
http://linked.open.../vavai/riv/strany	711-720
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QF4071)
http://linked.open...iv/cisloPeriodika	03
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Ryšánek, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / (nerozlišená součást)
http://linked.open...dnocenehoVysledku	580173
http://linked.open...ai/riv/idVysledku	RIV/60460709:_____/04:01:_3621
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ELISA, PCR, cereal products, food, monitoring, detection (en)
http://linked.open.../riv/klicoveSlovo	cereal products ELISA PCR detection monitoring food
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[8A837ABAD046]
http://linked.open...i/riv/nazevZdroje	Phytophaga 14
http://linked.open...in/vavai/riv/obor	GM
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated method of detection and monitoring of pest contamination of stored-grain and cereal products by physical, chemical, immunochemical and molecular techniques.
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	14
http://linked.open...iv/tvurceVysledku	Hubert, Jan Stejskal, Václav Ryšánek, Pavel Kudlíková, Iva Chalupníková, Julie
number of pages	9 (xsd:int)

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