RNA extraction method was optimalized for PLMVd detection. The best results were obtained using kit from Qiagen. PCR was also optimised. Primers designed on the basis of published PLMVd sequence give product 208 bp long.
RNA extraction method was optimalized for PLMVd detection. The best results were obtained using kit from Qiagen. PCR was also optimised. Primers designed on the basis of published PLMVd sequence give product 208 bp long. (en)