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Description
  • Isoflavones (3-phenyl chromones) are phytoalexins abundant in the Fabaceae, however their biosynthesis was described in over 20 other families, e.g. Rosaceae, Iridaceae, Asteraceae, Poaceae. Molecular biology data indicate occurrence of genes for isoflavonoid metabolic pathway even in higher number of families (1), although it is difficult to obtain the evidence of their biochemical activity. Recently, we have found isoflavonoids in the Rutaceae family (1). In our recent research, we combine immunochemical approaches and HPLC-MS. EXPERIMENTAL: Five immunoassays for early products of the isoflavonoid pathway (i.e. daidzein, formononetin, genistein and biochanin A and their glycosides) were adapted for phytochemical studies. Tested plant material (leaves from apical branches) was frozen immediately after collecting and kept in –20 °C until lyophilized. A limited number of samples were divided to two aliquots of which one was dried in laboratory temperature in shadow. Dry samples were pulverized and ext
  • Isoflavones (3-phenyl chromones) are phytoalexins abundant in the Fabaceae, however their biosynthesis was described in over 20 other families, e.g. Rosaceae, Iridaceae, Asteraceae, Poaceae. Molecular biology data indicate occurrence of genes for isoflavonoid metabolic pathway even in higher number of families (1), although it is difficult to obtain the evidence of their biochemical activity. Recently, we have found isoflavonoids in the Rutaceae family (1). In our recent research, we combine immunochemical approaches and HPLC-MS. EXPERIMENTAL: Five immunoassays for early products of the isoflavonoid pathway (i.e. daidzein, formononetin, genistein and biochanin A and their glycosides) were adapted for phytochemical studies. Tested plant material (leaves from apical branches) was frozen immediately after collecting and kept in –20 °C until lyophilized. A limited number of samples were divided to two aliquots of which one was dried in laboratory temperature in shadow. Dry samples were pulverized and ext (en)
  • Isoflavones (3-phenyl chromones) are phytoalexins abundant in the Fabaceae, however their biosynthesis was described in over 20 other families, e.g. Rosaceae, Iridaceae, Asteraceae, Poaceae. Molecular biology data indicate occurrence of genes for isoflavonoid metabolic pathway even in higher number of families (1), although it is difficult to obtain the evidence of their biochemical activity. Recently, we have found isoflavonoids in the Rutaceae family (1). In our recent research, we combine immunochemical approaches and HPLC-MS. EXPERIMENTAL: Five immunoassays for early products of the isoflavonoid pathway (i.e. daidzein, formononetin, genistein and biochanin A and their glycosides) were adapted for phytochemical studies. Tested plant material (leaves from apical branches) was frozen immediately after collecting and kept in –20 °C until lyophilized. A limited number of samples were divided to two aliquots of which one was dried in laboratory temperature in shadow. Dry samples were pulverized and ext (cs)
Title
  • Immunochemical screening of isoflavones in the Rutaceae family
  • Immunochemical screening of isoflavones in the Rutaceae family (en)
  • Immunochemický screening izoflavonů v čeledi Rutaceae (cs)
skos:prefLabel
  • Immunochemical screening of isoflavones in the Rutaceae family
  • Immunochemical screening of isoflavones in the Rutaceae family (en)
  • Immunochemický screening izoflavonů v čeledi Rutaceae (cs)
skos:notation
  • RIV/60460709:41610/04:9841!RIV/2005/GA0/416105/N
http://linked.open.../vavai/riv/strany
  • 215;216
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA525/03/0352)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 567330
http://linked.open...ai/riv/idVysledku
  • RIV/60460709:41610/04:9841
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • screening; isoflavones; immunoreactivity; Rutaceae (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [02B3DAA26EF1]
http://linked.open...v/mistoKonaniAkce
  • Olomouc, Czech Republic
http://linked.open...i/riv/mistoVydani
  • Olomouc, Czech Republic
http://linked.open...i/riv/nazevZdroje
  • 19th Meeting of Czech and Slovak Societies for Biochemistry and Molecular Biology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Kokoška, Ladislav
  • Lapčík, Oldřich
  • Koblovská, Radka
  • Klejdus, Bořivoj
  • Vítková, Michaela
  • Macková, Zuzana
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
http://purl.org/ne...btex#hasPublisher
  • Univerzita Palackého v Olomouci
https://schema.org/isbn
  • 80-244-0882-1
http://localhost/t...ganizacniJednotka
  • 41610
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