About: Selective growth-inhibitory effect of 8-hydroxyquinoline towards Clostridium difficile and Bifidobacterium longum subsp. longum in co-culture analyzed by flow cytometry combined with fluorescent in situ hybridization     Goto   Sponge   NotDistinct   Permalink

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  • The major risk factor for Clostridium difficile infection is the use of antibiotics owing to the disruption of the equilibrium of the host gut microbiota. To preserve the beneficial resident probiotic bacteria during infection treatment, the use of molecules with selective antibacterial activity enhances the efficacy by selectively removing C. difficile. One of them is the plant alkaloid 8-hydroxyquinoline (8HQ), which has been shown to selectively inhibit clostridia without repressing bifidobacteria. Selective antimicrobial activity is generally tested by culture techniques of individual bacterial strains. However, their main limitation is the inability to describe differential growth dynamics of more bacterial strains cultured together within the same experiment. In the present study we have combined fluorescent in situ hybridization and flow cytometry to describe the changes in active and non-active cells of a mixed culture formed by the opportunistic pathogen C. difficile CECT 531 and the benefic
  • The major risk factor for Clostridium difficile infection is the use of antibiotics owing to the disruption of the equilibrium of the host gut microbiota. To preserve the beneficial resident probiotic bacteria during infection treatment, the use of molecules with selective antibacterial activity enhances the efficacy by selectively removing C. difficile. One of them is the plant alkaloid 8-hydroxyquinoline (8HQ), which has been shown to selectively inhibit clostridia without repressing bifidobacteria. Selective antimicrobial activity is generally tested by culture techniques of individual bacterial strains. However, their main limitation is the inability to describe differential growth dynamics of more bacterial strains cultured together within the same experiment. In the present study we have combined fluorescent in situ hybridization and flow cytometry to describe the changes in active and non-active cells of a mixed culture formed by the opportunistic pathogen C. difficile CECT 531 and the benefic (en)
Title
  • Selective growth-inhibitory effect of 8-hydroxyquinoline towards Clostridium difficile and Bifidobacterium longum subsp. longum in co-culture analyzed by flow cytometry combined with fluorescent in situ hybridization
  • Selective growth-inhibitory effect of 8-hydroxyquinoline towards Clostridium difficile and Bifidobacterium longum subsp. longum in co-culture analyzed by flow cytometry combined with fluorescent in situ hybridization (en)
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  • Selective growth-inhibitory effect of 8-hydroxyquinoline towards Clostridium difficile and Bifidobacterium longum subsp. longum in co-culture analyzed by flow cytometry combined with fluorescent in situ hybridization
  • Selective growth-inhibitory effect of 8-hydroxyquinoline towards Clostridium difficile and Bifidobacterium longum subsp. longum in co-culture analyzed by flow cytometry combined with fluorescent in situ hybridization (en)
skos:notation
  • RIV/60460709:41340/14:66554!RIV15-MSM-41340___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
  • 63
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 44292
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  • RIV/60460709:41340/14:66554
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  • 8-Quinolinol, antimicrobial, Bifidobacterium, Clostridium, flow cytometry (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
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  • [1E0F95FBFEBB]
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  • JOURNAL OF MEDICAL MICROBIOLOGY
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • N
http://linked.open...iv/tvurceVysledku
  • Kokoška, Ladislav
  • Vlková, Eva
  • Nováková, Jitka
  • Musilová, Šárka
  • Moya, A.
  • D'Auria, Giuseppe
  • Dzunkova, M.
http://linked.open...ain/vavai/riv/wos
  • 0
issn
  • 0022-2615
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  • 41340
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