About: Comparative morphological and molecular identification of Haemonchus species in sheep

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A combined approach in the determination of Haemonchus nematodes from sheep was applied in this trial. Using selected morphological characters 90.2 % females and 84.2 % males of Haemonchus contortus and 9.8 % females and 15.8 % males of Haemonchus placei were identified. Although cluster analysis based on morphological identification clearly separated two Haemonchus species, H. contortus was exclusively detected in all specimens using restriction cleavage of the ITS-2 region with FspBI endonuclease as well as through the sequencing analysis. Because sheep from both farms have never had contact with other ruminants, and the farmers apply only closed flock turnover, we assume that only H. contortus mono-infection occurred on both farms. This opinion is also supported by molecular data. The most striking result of our study was the finding which indicates that the discriminant function is not able to accurately identify Haemonchus males at the species level. A combined approach in the determination of Haemonchus nematodes from sheep was applied in this trial. Using selected morphological characters 90.2 % females and 84.2 % males of Haemonchus contortus and 9.8 % females and 15.8 % males of Haemonchus placei were identified. Although cluster analysis based on morphological identification clearly separated two Haemonchus species, H. contortus was exclusively detected in all specimens using restriction cleavage of the ITS-2 region with FspBI endonuclease as well as through the sequencing analysis. Because sheep from both farms have never had contact with other ruminants, and the farmers apply only closed flock turnover, we assume that only H. contortus mono-infection occurred on both farms. This opinion is also supported by molecular data. The most striking result of our study was the finding which indicates that the discriminant function is not able to accurately identify Haemonchus males at the species level. (en)
Title	Comparative morphological and molecular identification of Haemonchus species in sheep Comparative morphological and molecular identification of Haemonchus species in sheep (en)
skos:prefLabel	Comparative morphological and molecular identification of Haemonchus species in sheep Comparative morphological and molecular identification of Haemonchus species in sheep (en)
skos:notation	RIV/60460709:41340/14:64703!RIV15-MZE-41340___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QI111A199)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Lukešová, D.
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / Fakulta tropického zemědělství
http://linked.open...dnocenehoVysledku	7948
http://linked.open...ai/riv/idVysledku	RIV/60460709:41340/14:64703
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	barber´s pole worm, spicule, discrimiant function, PCR-RFLP, sequencing, internal transcribed spacer (en)
http://linked.open.../riv/klicoveSlovo	internal transcribed spacer PCR-RFLP sequencing barber´s pole worm discrimiant function spicule
http://linked.open...odStatuVydavatele	PL - Polská republika
http://linked.open...ontrolniKodProRIV	[0478C8CE77F5]
http://linked.open...i/riv/nazevZdroje	Helminthologia
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Efficient system in sheep breeding parasitosis prevention
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	51
http://linked.open...iv/tvurceVysledku	Jankovská, Ivana Langrová, Iva Lukešová, Daniela Sedlák, Petr Vejl, Pavel Vadlejch, Jaroslav Čadková, Zuzana Salaba, Ondřej Vašek, Jakub
http://linked.open...ain/vavai/riv/wos	000338495400008
issn	0440-6605
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	41340

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