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  • Yacon (Smallanthus sonchifolius) is an Andean crop which is very regarded for its benefits for people suffering from diabetes or various digestive or renal disorders. Because no specific Smallanthus sonchifolius identification DNA markers are still known the paper demonstrates ITS regions to be able to detect and differentiate among yacon species and the potential for specific food authentification purposes is reported, too. The newly sequenced ITS of yacon accessions originated in Peru, Ecuador and Bolivia analyse provide the unique sequence site that differs from all of the other yacon species and is recognized by DraIII restriction endonuclease. Restriction cleavadge of the PCR amplified ITSs of the twenty-eight yacon accessions was performed and in all cases the recognition site was confirmed as a typical for Smallanthus sonchifolius . Based on the nucleotide specifity of Smallanthus sonchifolius, ITS sequence the PCR method combined with the restriction clevadge protocol wa
  • Yacon (Smallanthus sonchifolius) is an Andean crop which is very regarded for its benefits for people suffering from diabetes or various digestive or renal disorders. Because no specific Smallanthus sonchifolius identification DNA markers are still known the paper demonstrates ITS regions to be able to detect and differentiate among yacon species and the potential for specific food authentification purposes is reported, too. The newly sequenced ITS of yacon accessions originated in Peru, Ecuador and Bolivia analyse provide the unique sequence site that differs from all of the other yacon species and is recognized by DraIII restriction endonuclease. Restriction cleavadge of the PCR amplified ITSs of the twenty-eight yacon accessions was performed and in all cases the recognition site was confirmed as a typical for Smallanthus sonchifolius . Based on the nucleotide specifity of Smallanthus sonchifolius, ITS sequence the PCR method combined with the restriction clevadge protocol wa (en)
Title
  • A REVISED ITS NUCLEOTIDE SEQUENCE GIVES A SPECIFITY FOR Smallanthus sonchifolius (POEPP. AND ENDL.) AND ITS PRODUCTS IDENTIFICATION
  • A REVISED ITS NUCLEOTIDE SEQUENCE GIVES A SPECIFITY FOR Smallanthus sonchifolius (POEPP. AND ENDL.) AND ITS PRODUCTS IDENTIFICATION (en)
skos:prefLabel
  • A REVISED ITS NUCLEOTIDE SEQUENCE GIVES A SPECIFITY FOR Smallanthus sonchifolius (POEPP. AND ENDL.) AND ITS PRODUCTS IDENTIFICATION
  • A REVISED ITS NUCLEOTIDE SEQUENCE GIVES A SPECIFITY FOR Smallanthus sonchifolius (POEPP. AND ENDL.) AND ITS PRODUCTS IDENTIFICATION (en)
skos:notation
  • RIV/60460709:41340/13:60548!RIV14-MSM-41340___
http://linked.open...avai/riv/aktivita
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  • S
http://linked.open...iv/cisloPeriodika
  • 1
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http://linked.open...aciTvurceVysledku
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  • 59020
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  • RIV/60460709:41340/13:60548
http://linked.open...riv/jazykVysledku
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  • Smallantus sonchifolius (Poepp. and Endl.) H. Robinson, yacon, ITS, Dra III digestion, PCR (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [F987C3FD8534]
http://linked.open...i/riv/nazevZdroje
  • GENETIKA-BELGRADE
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 45
http://linked.open...iv/tvurceVysledku
  • Fernández Cusimamani, Eloy
  • Milella, Luigi
  • Ziarovska, Jana
http://linked.open...ain/vavai/riv/wos
  • 000320985900021
issn
  • 0534-0012
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http://localhost/t...ganizacniJednotka
  • 41340
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