About: Cumulus cell expansion, its role in oocyte biology and perspectives of measurement: A review

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Cumulus expansion of the cumulus-oocyte complex is necessary for meiotic maturation and acquiring developmental competence. Cumulus expansion is based on extracellular matrix synthesis by cumulus cells. Hyaluronic acid is the most abundant component of this extracellular matrix. Cumulus expansion takes place during meiotic oocyte maturation under in vivo and in vitro conditions. Quantification and measurement of cumulus expansion intensity is one possible method of determining oocyte quality and optimizing conditions for in vitro cultivation. Currently, subjective methods of expanded area and more exact cumulus expansion measurement by hyaluronic acid assessment are available. Among the methods of hyaluronic acid measurement is the use of radioactively labelled synthesis precursors. Alternatively, immunological and analytical methods, including enzyme-linked immunosorbent assay (ELISA), spectrophotometry, and high-performance liquid chromatography (HPLC) in UV light, could be utilized. The hi Cumulus expansion of the cumulus-oocyte complex is necessary for meiotic maturation and acquiring developmental competence. Cumulus expansion is based on extracellular matrix synthesis by cumulus cells. Hyaluronic acid is the most abundant component of this extracellular matrix. Cumulus expansion takes place during meiotic oocyte maturation under in vivo and in vitro conditions. Quantification and measurement of cumulus expansion intensity is one possible method of determining oocyte quality and optimizing conditions for in vitro cultivation. Currently, subjective methods of expanded area and more exact cumulus expansion measurement by hyaluronic acid assessment are available. Among the methods of hyaluronic acid measurement is the use of radioactively labelled synthesis precursors. Alternatively, immunological and analytical methods, including enzyme-linked immunosorbent assay (ELISA), spectrophotometry, and high-performance liquid chromatography (HPLC) in UV light, could be utilized. The hi (en)
Title	Cumulus cell expansion, its role in oocyte biology and perspectives of measurement: A review Cumulus cell expansion, its role in oocyte biology and perspectives of measurement: A review (en)
skos:prefLabel	Cumulus cell expansion, its role in oocyte biology and perspectives of measurement: A review Cumulus cell expansion, its role in oocyte biology and perspectives of measurement: A review (en)
skos:notation	RIV/60460709:41210/14:66087!RIV15-MZE-41210___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(QI101A166), S
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Nevoral, Jan Weingartová, Ivona Dvořáková, Markéta Jílek, František Krejčová, Tereza Orsák, Matyáš Zámostná, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / Fakulta agrobiologie, potravinových a přírodních zdrojů
http://linked.open...dnocenehoVysledku	9402
http://linked.open...ai/riv/idVysledku	RIV/60460709:41210/14:66087
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	oocyte, cumulus-oocyte complex, cumulus expansion, hyaluronic acid, spectrophotometry, high-performance liquid chromatography (en)
http://linked.open.../riv/klicoveSlovo	high-performance liquid chromatography oocyte spectrophotometry hyaluronic acid cumulus expansion cumulus-oocyte complex
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[404F808C8F6A]
http://linked.open...i/riv/nazevZdroje	Scientia Agriculturae Bohemica
http://linked.open...in/vavai/riv/obor	GG
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Biotechnology in pig husbandry and breeding
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	45
http://linked.open...iv/tvurceVysledku	Jílek, František Petr, Jaroslav Dvořáková, Markéta Krejčová, Tereza Orsák, Matyáš Vyskočilová, Alena Nevoral, Jan Weingartová, Ivona Klein, P. Zámostná, Kateřina
http://linked.open...ain/vavai/riv/wos	0
issn	1211-3174
number of pages	14 (xsd:int)
http://localhost/t...ganizacniJednotka	41210

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