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  • Bifidobacterium bifidum is a bacterial species exclusively found in the human intestinal tract. This species is becoming increasingly popular as a probiotic organism added to lyophilized products. In this study, porcine mucin was used as the sole carbon source for the selective enumeration of B. bifidum in probiotic food additives. Thirty-six bifidobacterial strains were cultivated in broth with mucin. Only 13 strains of B. bifidum utilized the mucin to produce acids. B. bifidum was selectively enumerated in eight probiotic food supplements using agar (MM agar) containing mupirocin (100 mg/L) and mucin (20 g/L) as the sole carbon source. MM agar was fully selective if the B. bifidum species was presented together with Bifidobacterium animalis subsp. lactis, Bifidobacteriumbreve, andBifidobacteriumlongumsubsp. longumspecies and with lactic acid bacteria (lactobacilli, streptococci). Isolated strains of B. bifidum were identified using biochemical, PCR, MALDI-TOF procedures and 16S rRNA gene s
  • Bifidobacterium bifidum is a bacterial species exclusively found in the human intestinal tract. This species is becoming increasingly popular as a probiotic organism added to lyophilized products. In this study, porcine mucin was used as the sole carbon source for the selective enumeration of B. bifidum in probiotic food additives. Thirty-six bifidobacterial strains were cultivated in broth with mucin. Only 13 strains of B. bifidum utilized the mucin to produce acids. B. bifidum was selectively enumerated in eight probiotic food supplements using agar (MM agar) containing mupirocin (100 mg/L) and mucin (20 g/L) as the sole carbon source. MM agar was fully selective if the B. bifidum species was presented together with Bifidobacterium animalis subsp. lactis, Bifidobacteriumbreve, andBifidobacteriumlongumsubsp. longumspecies and with lactic acid bacteria (lactobacilli, streptococci). Isolated strains of B. bifidum were identified using biochemical, PCR, MALDI-TOF procedures and 16S rRNA gene s (en)
Title
  • Mupirocin-mucin agar for selective enumeration of Bifidobacterium bifidum
  • Mupirocin-mucin agar for selective enumeration of Bifidobacterium bifidum (en)
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  • Mupirocin-mucin agar for selective enumeration of Bifidobacterium bifidum
  • Mupirocin-mucin agar for selective enumeration of Bifidobacterium bifidum (en)
skos:notation
  • RIV/60460709:41210/14:64624!RIV15-GA0-41210___
http://linked.open...avai/riv/aktivita
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  • I, P(GA13-08803S), S
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  • 191
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  • 31165
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  • RIV/60460709:41210/14:64624
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  • Probiotics Bifidobacterium bifidum Selective enumeration Isolation Feces (en)
http://linked.open.../riv/klicoveSlovo
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  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [37714C4D84C3]
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  • INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
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http://linked.open...UplatneniVysledku
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  • Killer, Jiří
  • Mrázek, Jakub
  • Vlková, Eva
  • Rada, Vojtěch
  • Bunešová, Věra
  • Pechar, Radko
  • Musilová, Šárka
  • Švejstil, Roman
  • Kmet, Vladimír
  • Parafati, Lucia
http://linked.open...ain/vavai/riv/wos
  • 000344823900005
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  • 0168-1605
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  • 41210
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