About: Exploitation of FTA cartridges for the sampling, long-term storage, and DNA-based analyses of plant-parasitic nematodes

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The use of DNA-based analyses in molecular plant nematology research has dramatically increased over recent decades. Therefore, the development and adaptation of simple, robust, and cost-effective DNA purification procedures are required to address these contemporary challenges. The solid-phase-based approach developed by Flinders Technology Associates (FTA) has been shown to be a powerful technology for the preparation of DNA from different biological materials, including blood, saliva, plant tissues, and various human and plant microbial pathogens. In this work, we demonstrate, for the first time, that this FTA-based technology is a valuable, low-cost, and time-saving approach for the sampling, long-term archiving, and molecular analysis of plant-parasitic nematodes. Despite the complex structure and anatomical organization of the multicellular bodies of nematodes, we report the successful and reliable DNA-based analysis of nematode high-copy and low-copy genes using the FTA technology. This was ac The use of DNA-based analyses in molecular plant nematology research has dramatically increased over recent decades. Therefore, the development and adaptation of simple, robust, and cost-effective DNA purification procedures are required to address these contemporary challenges. The solid-phase-based approach developed by Flinders Technology Associates (FTA) has been shown to be a powerful technology for the preparation of DNA from different biological materials, including blood, saliva, plant tissues, and various human and plant microbial pathogens. In this work, we demonstrate, for the first time, that this FTA-based technology is a valuable, low-cost, and time-saving approach for the sampling, long-term archiving, and molecular analysis of plant-parasitic nematodes. Despite the complex structure and anatomical organization of the multicellular bodies of nematodes, we report the successful and reliable DNA-based analysis of nematode high-copy and low-copy genes using the FTA technology. This was ac (en)
Title	Exploitation of FTA cartridges for the sampling, long-term storage, and DNA-based analyses of plant-parasitic nematodes Exploitation of FTA cartridges for the sampling, long-term storage, and DNA-based analyses of plant-parasitic nematodes (en)
skos:prefLabel	Exploitation of FTA cartridges for the sampling, long-term storage, and DNA-based analyses of plant-parasitic nematodes Exploitation of FTA cartridges for the sampling, long-term storage, and DNA-based analyses of plant-parasitic nematodes (en)
skos:notation	RIV/60460709:41210/14:64589!RIV15-MZE-41210___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QF4156)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Ryšánek, Pavel Zouhar, Miloslav Maňasová, Marie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / Fakulta agrobiologie, potravinových a přírodních zdrojů
http://linked.open...dnocenehoVysledku	16079
http://linked.open...ai/riv/idVysledku	RIV/60460709:41210/14:64589
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MELOIDOGYNE-INCOGNITA, DITYLENCHUS-DIPSACI, GENUS DITYLENCHUS, CYST-NEMATODE, RT-PCR, PATHOGENS, IDENTIFICATION, ANGUINIDAE, SEQUENCES, SEGMENTS (en)
http://linked.open.../riv/klicoveSlovo	IDENTIFICATION RT-PCR SEQUENCES ANGUINIDAE CYST-NEMATODE DITYLENCHUS-DIPSACI GENUS DITYLENCHUS MELOIDOGYNE-INCOGNITA PATHOGENS SEGMENTS
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[2ED989334B7E]
http://linked.open...i/riv/nazevZdroje	PHYTOPATHOLOGY
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of diagnostic methods for quarantine nematodes from genera Aphelenchoides, Bursaphelenchus, Xiphinema and Radopholus.
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	104
http://linked.open...iv/tvurceVysledku	Douda, Ondřej Marek, Martin Zouhar, Miloslav Ryšánek, Pavel Maňasová, Marie
http://linked.open...ain/vavai/riv/wos	00033185940001
issn	0031-949X
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	41210

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