About: The effect of protein kinase C activator and nitric oxide donor on oocyte activation and cortical granule exocytosis in porcine eggs

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About: The effect of protein kinase C activator and nitric oxide donor on oocyte activation and cortical granule exocytosis in porcine eggs Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Nitric oxide (NO) and protein kinase C (PKC) are involved in the activation of mammalian oocytes, although their role in the exit from the metaphase II stage and cortical granules (CG) exocytosis is still not fully understood. The aim of this study was to verify whether NO-donor together with specific PKC-activators induce the complete activation of porcine oocytes assessed as meiosis resumption and a cortical reaction. Pig maturated oocytes were treated with the NO-donor S-nitroso-N-acetylpenicillamine (SNAP) or PKC-activators such as phorbol-12-myristate-13-acetate (PMA), 1-oleyl-2-acetyl-sn-glycerol (OAG) and L-?-phosphatidylinositol-3,4,5-trisphosphate dipalmitoyl heptaammonium salt (DPAM). To study the combined effect of NO-donor and PKC-activators, aliquots of oocytes were also incubated with SNAP together with PKC-activators (SNAP-DPAM, SNAP-OAG and SNAP-PMA groups). The results showed that 0% of control oocytes reassumed meiosis. However, both the PKC-activators (DPAM 44.0 ± 10.0%, OAG 63.3 ± Nitric oxide (NO) and protein kinase C (PKC) are involved in the activation of mammalian oocytes, although their role in the exit from the metaphase II stage and cortical granules (CG) exocytosis is still not fully understood. The aim of this study was to verify whether NO-donor together with specific PKC-activators induce the complete activation of porcine oocytes assessed as meiosis resumption and a cortical reaction. Pig maturated oocytes were treated with the NO-donor S-nitroso-N-acetylpenicillamine (SNAP) or PKC-activators such as phorbol-12-myristate-13-acetate (PMA), 1-oleyl-2-acetyl-sn-glycerol (OAG) and L-?-phosphatidylinositol-3,4,5-trisphosphate dipalmitoyl heptaammonium salt (DPAM). To study the combined effect of NO-donor and PKC-activators, aliquots of oocytes were also incubated with SNAP together with PKC-activators (SNAP-DPAM, SNAP-OAG and SNAP-PMA groups). The results showed that 0% of control oocytes reassumed meiosis. However, both the PKC-activators (DPAM 44.0 ± 10.0%, OAG 63.3 ± (en)
Title	The effect of protein kinase C activator and nitric oxide donor on oocyte activation and cortical granule exocytosis in porcine eggs The effect of protein kinase C activator and nitric oxide donor on oocyte activation and cortical granule exocytosis in porcine eggs (en)
skos:prefLabel	The effect of protein kinase C activator and nitric oxide donor on oocyte activation and cortical granule exocytosis in porcine eggs The effect of protein kinase C activator and nitric oxide donor on oocyte activation and cortical granule exocytosis in porcine eggs (en)
skos:notation	RIV/60460709:41210/13:59827!RIV14-MSM-41210___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GAP502/10/0794), Z(MSM6046070901)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Tůmová, Lenka Sedmíková, Markéta
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / Fakulta agrobiologie, potravinových a přírodních zdrojů
http://linked.open...dnocenehoVysledku	71694
http://linked.open...ai/riv/idVysledku	RIV/60460709:41210/13:59827
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	protein kinase C, nitric oxide, pig oocyte, activation, cortical granules (en)
http://linked.open.../riv/klicoveSlovo	activation cortical granules pig oocyte protein kinase C nitric oxide
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[205F6DF8F975]
http://linked.open...i/riv/nazevZdroje	Animal
http://linked.open...in/vavai/riv/obor	GG
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Acquisition of meiotic competence in pig oocytes - the effects of protein phosphatase calcineurin
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	7
http://linked.open...iv/tvurceVysledku	Petr, Jaroslav Tůmová, Lenka Sedmíková, Markéta Romar, Raquel
http://linked.open...ain/vavai/riv/wos	000313218900012
http://linked.open...n/vavai/riv/zamer	Setrvalé zemědělství, kvalita zemědělské produkce, krajinné a přírodní zdroje.
issn	1751-7311
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	41210

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