About: Superoxide dismutase isoelectric focusing patterns as a tool to differentiate pathotypes of Globodera spp.

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About: Superoxide dismutase isoelectric focusing patterns as a tool to differentiate pathotypes of Globodera spp. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Isoelectric focusing was used to separate proteins from cyst extracts of potato cyst nematode populations. Cyst extracts from standard populations of Globodera rostochiensis pathotypes Ro1, Ro2, Ro3, Ro2/3, Ro4 and Ro5 and G. pallida pathotypes Pa2 and Pa3, were loaded on isoelectric focusing gels. Gels were stained for superoxide dismutase, esterase, and glucose-6-phosphate isomerase. Twelve bands of SOD activity were detected. A cluster analysis was carried out based on a data matrix that reported the presence or absence of SOD bands on the isozyme electrophoresis patterns (IEPs). Globodera spp. were clearly distinguished and, within G. rostochiensis, Ro2 and Ro4 shared a high level of similarity, respectively, with Ro3 and Ro5; moreover, Ro1 could be clearly distinguished from Ro2/3 and Ro4/5. G.pallida Pa2 and Pa3 also shared a high level of similarity. In contrast, esterase and GPI IEPs did not discriminate among G. rostochiensis standard pathotypes. Isoelectric focusing was used to separate proteins from cyst extracts of potato cyst nematode populations. Cyst extracts from standard populations of Globodera rostochiensis pathotypes Ro1, Ro2, Ro3, Ro2/3, Ro4 and Ro5 and G. pallida pathotypes Pa2 and Pa3, were loaded on isoelectric focusing gels. Gels were stained for superoxide dismutase, esterase, and glucose-6-phosphate isomerase. Twelve bands of SOD activity were detected. A cluster analysis was carried out based on a data matrix that reported the presence or absence of SOD bands on the isozyme electrophoresis patterns (IEPs). Globodera spp. were clearly distinguished and, within G. rostochiensis, Ro2 and Ro4 shared a high level of similarity, respectively, with Ro3 and Ro5; moreover, Ro1 could be clearly distinguished from Ro2/3 and Ro4/5. G.pallida Pa2 and Pa3 also shared a high level of similarity. In contrast, esterase and GPI IEPs did not discriminate among G. rostochiensis standard pathotypes. (en)
Title	Superoxide dismutase isoelectric focusing patterns as a tool to differentiate pathotypes of Globodera spp. Superoxide dismutase isoelectric focusing patterns as a tool to differentiate pathotypes of Globodera spp. (en)
skos:prefLabel	Superoxide dismutase isoelectric focusing patterns as a tool to differentiate pathotypes of Globodera spp. Superoxide dismutase isoelectric focusing patterns as a tool to differentiate pathotypes of Globodera spp. (en)
skos:notation	RIV/60460709:41210/10:49425!RIV11-MSM-41210___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6046070901)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Zouhar, Miloslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / Fakulta agrobiologie, potravinových a přírodních zdrojů
http://linked.open...dnocenehoVysledku	291066
http://linked.open...ai/riv/idVysledku	RIV/60460709:41210/10:49425
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	diagnostics, isoelectric focusing, nematodes, pathotypes, potato cyst, SOD (en)
http://linked.open.../riv/klicoveSlovo	isoelectric focusing diagnostics nematodes SOD potato cyst pathotypes
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[29143B79F424]
http://linked.open...i/riv/nazevZdroje	Nematology
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	12
http://linked.open...iv/tvurceVysledku	Zouhar, Miloslav Greco, N. Molinari, Sergio
http://linked.open...ain/vavai/riv/wos	000284665900009
http://linked.open...n/vavai/riv/zamer	Setrvalé zemědělství, kvalita zemědělské produkce, krajinné a přírodní zdroje.
issn	1388-5545
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	41210

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