About: Biological characterization and variability in the coat protein gene of an isolate of Apricot latent virus.

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About: Biological characterization and variability in the coat protein gene of an isolate of Apricot latent virus. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Virus-free accessions of peach, apricot, cherry and plum were graft-inoculated in 2004 with buds from a peach tree cv. Missour infected by Apricot latent virus (ApLV), and then analysed by RT-PCR using a specific primer set. The expected DNA fragment of 200 bp in size was amplified from 33 different Prunus sources, thus extending the woody host range of ApLV to new cultivars of Prunus persica, P. avium, P. armeniaca and for the first time to European (Prunus domestica) and Japanese (P. salicina) plum cultivars. Several peach cultivars and apricot cv. Tirynthos were symptomatic. To determine the best source material for ApLV detection, RT-PCR assays were performed during the growing season. ApLV was homogeneously distributed in flowers, leaves, petioles, barks and fruits of peach and apricot but was detected only in leaves of cherry and plum. The genetic variability of the ApLV coat protein gene from graft-inoculated hosts and corresponding sequences from databases was 63.5-100%. Virus-free accessions of peach, apricot, cherry and plum were graft-inoculated in 2004 with buds from a peach tree cv. Missour infected by Apricot latent virus (ApLV), and then analysed by RT-PCR using a specific primer set. The expected DNA fragment of 200 bp in size was amplified from 33 different Prunus sources, thus extending the woody host range of ApLV to new cultivars of Prunus persica, P. avium, P. armeniaca and for the first time to European (Prunus domestica) and Japanese (P. salicina) plum cultivars. Several peach cultivars and apricot cv. Tirynthos were symptomatic. To determine the best source material for ApLV detection, RT-PCR assays were performed during the growing season. ApLV was homogeneously distributed in flowers, leaves, petioles, barks and fruits of peach and apricot but was detected only in leaves of cherry and plum. The genetic variability of the ApLV coat protein gene from graft-inoculated hosts and corresponding sequences from databases was 63.5-100%. (en)
Title	Biological characterization and variability in the coat protein gene of an isolate of Apricot latent virus. Biological characterization and variability in the coat protein gene of an isolate of Apricot latent virus. (en)
skos:prefLabel	Biological characterization and variability in the coat protein gene of an isolate of Apricot latent virus. Biological characterization and variability in the coat protein gene of an isolate of Apricot latent virus. (en)
skos:notation	RIV/60460709:41210/10:49040!RIV11-MSM-41210___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QG60123), Z(MSM6046070901)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Ryšánek, Pavel Zouhar, Miloslav Grimová, Lenka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / Fakulta agrobiologie, potravinových a přírodních zdrojů
http://linked.open...dnocenehoVysledku	248897
http://linked.open...ai/riv/idVysledku	RIV/60460709:41210/10:49040
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ApLV, stone fruit, symptoms, RT-PCR, sequence analysis. (en)
http://linked.open.../riv/klicoveSlovo	RT-PCR stone fruit ApLV sequence analysis. symptoms
http://linked.open...odStatuVydavatele	IT - Italská republika
http://linked.open...ontrolniKodProRIV	[2F7E0C7CD8B4]
http://linked.open...i/riv/nazevZdroje	Journal of Plant Pathology
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Research and innovation of diagnostic procedures for economically significant, controlled and quarantine plant pathogenic organisms for the certification program of fruit trees with emphasis on molecular methods
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	92
http://linked.open...iv/tvurceVysledku	Zouhar, Miloslav Ryšánek, Pavel Grimová, Lenka Bazzoni, Alessandra Minafra, Angelantonio Palmisano, Francesco Savino, Vito
http://linked.open...ain/vavai/riv/wos	000276175100011
http://linked.open...n/vavai/riv/zamer	Setrvalé zemědělství, kvalita zemědělské produkce, krajinné a přírodní zdroje.
issn	1125-4653
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	41210

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