About: Quantification of Tilletia caries and Tilletia controversa mycelium in wheat apical meristem by real-time PCR

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Quantification of Tilletia caries and Tilletia controversa mycelium in wheat apical meristem by real-time PCR Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In the Czech Republic, three closely related species of the genus Tilletia belong to pathogens that cause significant losses of wheat crops by replacing grains with a mass of teliospores. A quantitative real-time qPCR assay using SYBR Green I has been developed to quantify the amount of T. caries and T. controversa mycelium in apical meristems of different wheat varieties. The real-time PCR reaction was validated by evaluating selected extraction methods, examining the specificity of designed target-specific IGS primers and verifying the optimised amplification reaction on naturally infected wheat plants. The PCR detection limit for the specific identification of fungal DNA was 0.22 ng of mycelium, and the negative correlation between the target DNA quantity and cycle threshold (Ct) was high with a coefficient of determination of R2 = 0.992. The developed method was used to quantify pathogens mycelium in five wheat varieties in the range from 0.34 ng to 15 µg per one growing tip. In the Czech Republic, three closely related species of the genus Tilletia belong to pathogens that cause significant losses of wheat crops by replacing grains with a mass of teliospores. A quantitative real-time qPCR assay using SYBR Green I has been developed to quantify the amount of T. caries and T. controversa mycelium in apical meristems of different wheat varieties. The real-time PCR reaction was validated by evaluating selected extraction methods, examining the specificity of designed target-specific IGS primers and verifying the optimised amplification reaction on naturally infected wheat plants. The PCR detection limit for the specific identification of fungal DNA was 0.22 ng of mycelium, and the negative correlation between the target DNA quantity and cycle threshold (Ct) was high with a coefficient of determination of R2 = 0.992. The developed method was used to quantify pathogens mycelium in five wheat varieties in the range from 0.34 ng to 15 µg per one growing tip. (en)
Title	Quantification of Tilletia caries and Tilletia controversa mycelium in wheat apical meristem by real-time PCR Quantification of Tilletia caries and Tilletia controversa mycelium in wheat apical meristem by real-time PCR (en)
skos:prefLabel	Quantification of Tilletia caries and Tilletia controversa mycelium in wheat apical meristem by real-time PCR Quantification of Tilletia caries and Tilletia controversa mycelium in wheat apical meristem by real-time PCR (en)
skos:notation	RIV/60460709:41210/10:48822!RIV11-MSM-41210___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QH71105), Z(MSM6046070901)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Prokinová, Evženie Mazáková, Jana Ryšánek, Pavel Zouhar, Miloslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / Fakulta agrobiologie, potravinových a přírodních zdrojů
http://linked.open...dnocenehoVysledku	283619
http://linked.open...ai/riv/idVysledku	RIV/60460709:41210/10:48822
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Tilletia spp., real-time PCR, apical meristem, absolute quantification (en)
http://linked.open.../riv/klicoveSlovo	absolute quantification apical meristem real-time PCR Tilletia spp.
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[3CF668C47668]
http://linked.open...i/riv/nazevZdroje	Plant Protection Science (Ochrana rostlin)
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Tilletia species on winter wheat
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	46
http://linked.open...iv/tvurceVysledku	Mazáková, Jana Váňová, Marie Prokinová, Evženie Zouhar, Miloslav Ryšánek, Pavel
http://linked.open...ain/vavai/riv/wos	0
http://linked.open...n/vavai/riv/zamer	Setrvalé zemědělství, kvalita zemědělské produkce, krajinné a přírodní zdroje.
issn	1212-2580
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	41210

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software