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Description
  • Peach latent mosaic viroid (PLMVd) is a widespread pathogen of stone fruit trees in some European and Mediterranean countries, and also in Northern America. To access the presence of the viroid in Egypt, a survey was conducted covering five commercial peach orchards in El Khatatba region in Al Minufiya governorate. During 2003-2004, 73 peach trees (cv. Florida grafted on Nemagard rootstock) were visually inspected and sampled. No symptoms characteristic of PLMVd infection as mosaic, delayed vegetation or fruit suture cracking were observed. All samples were tested for the presence of PLMVd using dot-blot hybridization and reverse transcription-polymerase chain reaction (RT-PCR). Aliquots (5 µl) of total nucleic acids, extracted from about 2 mg of leaf tissues, were spotted onto positively charged nylon membranes and hybridized under stringent conditions using a digoxigenin-labeled riboprobe (1). The extracts (1 µl) also were used in RT-PCR as described by Loreti et al. (2). Only one of the 73 peac
  • Peach latent mosaic viroid (PLMVd) is a widespread pathogen of stone fruit trees in some European and Mediterranean countries, and also in Northern America. To access the presence of the viroid in Egypt, a survey was conducted covering five commercial peach orchards in El Khatatba region in Al Minufiya governorate. During 2003-2004, 73 peach trees (cv. Florida grafted on Nemagard rootstock) were visually inspected and sampled. No symptoms characteristic of PLMVd infection as mosaic, delayed vegetation or fruit suture cracking were observed. All samples were tested for the presence of PLMVd using dot-blot hybridization and reverse transcription-polymerase chain reaction (RT-PCR). Aliquots (5 µl) of total nucleic acids, extracted from about 2 mg of leaf tissues, were spotted onto positively charged nylon membranes and hybridized under stringent conditions using a digoxigenin-labeled riboprobe (1). The extracts (1 µl) also were used in RT-PCR as described by Loreti et al. (2). Only one of the 73 peac (en)
  • Peach latent mosaic viroid (PLMVd) is a widespread pathogen of stone fruit trees in some European and Mediterranean countries, and also in Northern America. To access the presence of the viroid in Egypt, a survey was conducted covering five commercial peach orchards in El Khatatba region in Al Minufiya governorate. During 2003-2004, 73 peach trees (cv. Florida grafted on Nemagard rootstock) were visually inspected and sampled. No symptoms characteristic of PLMVd infection as mosaic, delayed vegetation or fruit suture cracking were observed. All samples were tested for the presence of PLMVd using dot-blot hybridization and reverse transcription-polymerase chain reaction (RT-PCR). Aliquots (5 µl) of total nucleic acids, extracted from about 2 mg of leaf tissues, were spotted onto positively charged nylon membranes and hybridized under stringent conditions using a digoxigenin-labeled riboprobe (1). The extracts (1 µl) also were used in RT-PCR as described by Loreti et al. (2). Only one of the 73 peac (cs)
Title
  • First report of Peach latent mosaic viroid infecting peach in Egypt
  • First report of Peach latent mosaic viroid infecting peach in Egypt (en)
  • První nález viroidu latentní mozaiky broskvoně v Egyptě (cs)
skos:prefLabel
  • First report of Peach latent mosaic viroid infecting peach in Egypt
  • First report of Peach latent mosaic viroid infecting peach in Egypt (en)
  • První nález viroidu latentní mozaiky broskvoně v Egyptě (cs)
skos:notation
  • RIV/60460709:41210/08:26261!RIV09-MZE-41210___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(QG60123), Z(MSM6046070901)
http://linked.open...iv/cisloPeriodika
  • 92
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 368111
http://linked.open...ai/riv/idVysledku
  • RIV/60460709:41210/08:26261
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • PLMVd; peach; Egypt (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [4B7693D693A4]
http://linked.open...i/riv/nazevZdroje
  • Plant Disease
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 4
http://linked.open...iv/tvurceVysledku
  • Ryšánek, Pavel
  • Hassan, M.
  • Alioto, D.
  • Malfitano, M.
http://linked.open...n/vavai/riv/zamer
issn
  • 0191-2917
number of pages
http://localhost/t...ganizacniJednotka
  • 41210
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