About: Effects of genistein and genistin on in vitro maturation of pig oocytes

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The objective of the study was to verify the hypothesis that GEN (genistein – phytoestrogen and an inhibitor of tyrosine protein kinase – TPK) effects on pig oocyte maturation and cumular cell expansion under in vitro conditions are connected with its estrogenic activity. Oocytes were cultivated for 24 hours up to the stage of the first meiotic metaphase (MI). Three different doses of GEN (13, 40, 80 µg/ml of medium) and also three doses of GIN, genistin, an analogue of GEN without effects on TPK, (80, 160 and 240 µg/ml of medium) were tested. To verify the reversibility of GEN effects, the oocytes were first cultivated for 24 hours with 80 µg of GEN per 1 ml of medium and then for another 24 hours without any GEN. GEN blocked pig oocyte maturation at the stage of the germinal vesicle (GV), depending on the dose. After rinsing out the GEN the oocyte maturation recovered, but with abnormalities (32%). GIN in a concentration of 80 µg/ml of medium induced a significant blockage at the GV stage The objective of the study was to verify the hypothesis that GEN (genistein – phytoestrogen and an inhibitor of tyrosine protein kinase – TPK) effects on pig oocyte maturation and cumular cell expansion under in vitro conditions are connected with its estrogenic activity. Oocytes were cultivated for 24 hours up to the stage of the first meiotic metaphase (MI). Three different doses of GEN (13, 40, 80 µg/ml of medium) and also three doses of GIN, genistin, an analogue of GEN without effects on TPK, (80, 160 and 240 µg/ml of medium) were tested. To verify the reversibility of GEN effects, the oocytes were first cultivated for 24 hours with 80 µg of GEN per 1 ml of medium and then for another 24 hours without any GEN. GEN blocked pig oocyte maturation at the stage of the germinal vesicle (GV), depending on the dose. After rinsing out the GEN the oocyte maturation recovered, but with abnormalities (32%). GIN in a concentration of 80 µg/ml of medium induced a significant blockage at the GV stage (en)
Title	Effects of genistein and genistin on in vitro maturation of pig oocytes Effects of genistein and genistin on in vitro maturation of pig oocytes (en)
skos:prefLabel	Effects of genistein and genistin on in vitro maturation of pig oocytes Effects of genistein and genistin on in vitro maturation of pig oocytes (en)
skos:notation	RIV/60460709:41210/08:25732!RIV13-MSM-41210___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GD523/03/H076), Z(MSM6046070901), Z(MZE0002701401)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Jílek, František Rajmon, Radko Klabanová, Petra Vodková, Zuzana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / Fakulta agrobiologie, potravinových a přírodních zdrojů
http://linked.open...dnocenehoVysledku	365391
http://linked.open...ai/riv/idVysledku	RIV/60460709:41210/08:25732
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	phytoestrogen, isoflavone, oocyte maturation, pig, estrogenic effect, tyrosine protein kinase (en)
http://linked.open.../riv/klicoveSlovo	oocyte maturation isoflavone phytoestrogen tyrosine protein kinase pig estrogenic effect
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[BB236A3F3CAE]
http://linked.open...i/riv/nazevZdroje	Czech Journal of Animal Science
http://linked.open...in/vavai/riv/obor	GG
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Enhancing of Methodological Level and Theoretical Education of Students of the Ph. D. Program 4103V - Animal Husbandry - a Perspective Field General Animal Husb
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	53
http://linked.open...iv/tvurceVysledku	Jílek, František Petr, Jaroslav Rajmon, Radko Vodková, Zuzana Klabanová, Petra
http://linked.open...n/vavai/riv/zamer	Setrvalé zemědělství, kvalita zemědělské produkce, krajinné a přírodní zdroje. Od rostoucího oocytu k produkci embryí in vitro a embryonálním kmenovým buňkám: Vývojové modely a strategie produkce zárodečných, embryonálních a somatických buněk in vitro v kontextu s jejich využitím v programu zachování genetických živočišných zdrojů
issn	1212-1819
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	41210

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